Benson S, Shapiro J
J Bacteriol. 1978 Jul;135(1):278-80. doi: 10.1128/jb.135.1.278-280.1978.
We readily isolated insertions of the carbenicillin resistance element Tn401 into the TOL plasmid in Pseudomonas putida. Hybrid TOL::Tn401 plasmids stably express the Cbr phenotype in Pseudomonas aeruginosa and Escherichia coli. Whereas the replicative and conjugative functions are expressed in both hosts, the ability to grow on m-toluate is only expressed in the Pseudomonas species.
我们很容易地从恶臭假单胞菌中分离出羧苄青霉素抗性元件Tn401插入到TOL质粒中的情况。杂合的TOL::Tn401质粒在铜绿假单胞菌和大肠杆菌中稳定表达Cbr表型。虽然复制和接合功能在两种宿主中都有表达,但在间甲苯酸盐上生长的能力仅在假单胞菌属物种中表达。