假单胞菌中降解性质粒聚集体的解离
Dissociation of a degradative plasmid aggregate in Pseudomonas.
作者信息
Chakrabarty A M
出版信息
J Bacteriol. 1974 Jun;118(3):815-20. doi: 10.1128/jb.118.3.815-820.1974.
Abstract
The infectious plasmid OCT, which specifies a set of dissimilatory enzymes responsible for the degradation of n-octane, has been shown to be an aggregate of a noninfectious OCT plasmid and an infectious plasmid with sex factor activity. The infectious plasmid, which can be eliminated from the cells of Pseudomonas putida by mitomycin C treatment without loss of the OCT plasmid and vice versa, has been designated as factor K. The infectious plasmid (factor K) is not only responsible for the mobilization of OCT, but also mobilizes chromosomal genes at a frequency of 10(-2) to 10(-3) per donor cell. Whereas OCT is incompatible with another degradative plasmid, CAM, factor K appears to be compatible with it.
摘要
可编码一组负责正辛烷降解的异化酶的感染性质粒OCT，已被证明是一种非感染性OCT质粒与一种具有性因子活性的感染性质粒的聚集体。通过丝裂霉素C处理可从恶臭假单胞菌细胞中消除该感染性质粒，而不损失OCT质粒，反之亦然，该感染性质粒已被命名为K因子。感染性质粒（K因子）不仅负责OCT的转移，还以每个供体细胞10^(-2)至10^(-3)的频率转移染色体基因。虽然OCT与另一种降解性质粒CAM不相容，但K因子似乎与之相容。
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