Paturi Gunaranjan, Butts Christine A, Bentley-Hewitt Kerry L, McGhie Tony K, Saleh Zaid S, McLeod Andrew
The New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland, 1142, New Zealand.
Phytother Res. 2014 Dec;28(12):1846-54. doi: 10.1002/ptr.5210. Epub 2014 Jul 28.
The protective role of two apple polyphenol extracts, Douglas-FB (FB) and Douglas-EF (EF), on gastric mucosal damage following aspirin ingestion was investigated in healthy rats. Polyphenol content of the apple extracts varied, with the EF extract having 20% w/w polyphenols and a high proportion of flavanols as epicatechin and procyanidin, whereas the FB extract comprised 12% w/w polyphenols, which were mostly flavonols as quercetin glycosides. Male Sprague-Dawley rats were allocated to control, FB and EF groups and fed the experimental diet during the 10-day trial. Control treatment rats received 1 mL of deionised water, whereas apple polyphenol treatment group rats, FB and EF received a concentration of 10(-2) m polyphenols in 1 mL deionised water daily via oral gavage. At the end of 10-day feeding period, rats were fasted overnight, and the following morning, aspirin (200 mg/kg) was given by oral gavage. Four hours after aspirin administration, the animals were euthanised, and samples taken for analysis. Both apple polyphenol extracts significantly reduced the ulcer area, ulcer lesion index and gastric injury score. The glutathione in gastric mucosa was increased significantly in rats given FB apple extract. Despite their different polyphenol compositions, FB and EF apple extracts assisted in protecting the gastric mucosa following acute aspirin administration in rats.
在健康大鼠中研究了两种苹果多酚提取物，道格拉斯-FB（FB）和道格拉斯-EF（EF）对阿司匹林摄入后胃黏膜损伤的保护作用。苹果提取物中的多酚含量各不相同，EF提取物含有20%（w/w）的多酚，且黄烷醇（如表儿茶素和原花青素）比例较高，而FB提取物含有12%（w/w）的多酚，主要是黄酮醇（如槲皮素糖苷）。将雄性Sprague-Dawley大鼠分为对照组、FB组和EF组，并在为期10天的试验期间给予实验饮食。对照处理的大鼠接受1mL去离子水，而苹果多酚处理组的大鼠，FB组和EF组每天通过口服灌胃给予浓度为10⁻² m的多酚于1mL去离子水中。在10天喂养期结束时，大鼠禁食过夜，次日早晨通过口服灌胃给予阿司匹林（200mg/kg）。阿司匹林给药4小时后，对动物实施安乐死，并采集样本进行分析。两种苹果多酚提取物均显著降低了溃疡面积、溃疡病变指数和胃损伤评分。给予FB苹果提取物的大鼠胃黏膜中的谷胱甘肽显著增加。尽管FB和EF苹果提取物的多酚组成不同，但它们在大鼠急性给予阿司匹林后有助于保护胃黏膜。
