The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North, 4442, New Zealand.
School of Veterinary Science, Massey University, Palmerston North, New Zealand.
Sci Rep. 2020 Aug 3;10(1):13055. doi: 10.1038/s41598-020-70006-0.
Kiwifruit (KF) contains bioactive compounds with potential anti-inflammatory properties. In this study, we investigated the protective effects of KF on gastric and duodenal damage induced by soluble aspirin in healthy rats. Sixty-four male Sprague Dawley rats were allocated to eight experimental treatments (n = 8) and the experimental diets were fed for 14 days ad libitum. The experimental diets were 20% fresh pureed KF (green-fleshed and gold-fleshed) or 10% glucose solution (control diet). A positive anti-inflammatory control treatment (ranitidine) was included. At the end of the 14-day feeding period, the rats were fasted overnight, and the following morning soluble aspirin (400 mg/kg aspirin) or water (control) was administered by oral gavage. Four hours after aspirin administration, the rats were euthanized and samples taken for analysis. We observed no significant ulcer formation or increase in infiltration of the gastric mucosal inflammatory cells in the rats with the aspirin treatment. Despite this, there were significant changes in gene expression, such as in the duodenum of aspirin-treated rats fed green KF where there was increased expression of inflammation-related genes NOS2 and TNF-alpha. We also observed that gold and green KF diets had a number of contrasting effects on genes related to inflammation and gastro-protective effects.
奇异果（KF）含有具有潜在抗炎特性的生物活性化合物。在这项研究中，我们研究了 KF 对健康大鼠因可溶性阿司匹林引起的胃和十二指肠损伤的保护作用。64 只雄性 Sprague Dawley 大鼠被分配到 8 个实验处理组（n=8），并进行 14 天的自由进食实验饮食。实验饮食为 20%新鲜果泥奇异果（绿肉和金肉）或 10%葡萄糖溶液（对照饮食）。还包括阳性抗炎对照处理（雷尼替丁）。在 14 天喂养期结束时，大鼠禁食过夜，第二天早上通过口服灌胃给予可溶性阿司匹林（400mg/kg 阿司匹林）或水（对照）。在给予阿司匹林 4 小时后，对大鼠进行安乐死并取样进行分析。我们观察到阿司匹林治疗的大鼠胃黏膜无明显溃疡形成或炎症细胞浸润增加。尽管如此，基因表达仍发生了显著变化，例如在接受阿司匹林治疗的大鼠的十二指肠中，与炎症相关的基因 NOS2 和 TNF-alpha 的表达增加。我们还观察到，金奇异果和绿奇异果饮食对与炎症和胃保护作用相关的基因有许多相反的影响。
