Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA.
Opt Lett. 2013 Aug 1;38(15):2683-6. doi: 10.1364/OL.38.002683.
We developed random-access optical-resolution photoacoustic microscopy using a digital micromirror device. This system can rapidly scan arbitrarily shaped regions of interest within a 40 μm×40 μm imaging area with a lateral resolution of 3.6 μm. To identify a region of interest, a global structural image is first acquired, then the selected region is scanned. The random-access ability was demonstrated by imaging two static samples, a carbon fiber cross and a monolayer of red blood cells, with an acquisition rate up to 4 kHz. The system was then used to monitor blood flow in vivo in real time within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 9.2 times.
我们使用数字微镜器件开发了随机访问光分辨率光声显微镜。该系统可以快速扫描 40μm×40μm 成像区域内任意形状的感兴趣区域，具有 3.6μm 的横向分辨率。为了识别感兴趣的区域，首先获取全局结构图像，然后扫描所选区域。通过对两个静态样本（碳纤维十字和单层红细胞）进行成像，证明了该系统具有高达 4kHz 的采集率的随机访问能力。然后，该系统用于实时监测小鼠耳朵中用户选择的毛细血管内的血流。通过仅对感兴趣的毛细血管成像，帧率提高了 9.2 倍。
