Strathmann M, Wilkie T M, Simon M I
Division of Biology, California Institute of Technology, Pasadena 91125.
Proc Natl Acad Sci U S A. 1989 Oct;86(19):7407-9. doi: 10.1073/pnas.86.19.7407.
Biochemical analysis has revealed a number of guanine nucleotide-binding regulatory proteins (G proteins) that mediate signal transduction in mammalian systems. Characterization of their cDNAs uncovered a family of proteins with regions of highly conserved amino acid sequence. To examine the extent of diversity of the G protein family, we used the polymerase chain reaction to detect additional gene products in mouse brain and spermatid RNA that share these conserved regions. Sequences corresponding to six of the eight known G protein alpha subunits were obtained. In addition, we found sequences corresponding to five newly discovered alpha subunits. Our results suggest that the complexity of the G protein family is much greater than previously suspected.
生化分析揭示了许多鸟嘌呤核苷酸结合调节蛋白（G蛋白），它们在哺乳动物系统中介导信号转导。对其cDNA的表征发现了一类蛋白质，其氨基酸序列具有高度保守区域。为了研究G蛋白家族的多样性程度，我们使用聚合酶链反应来检测小鼠脑和精子细胞RNA中共享这些保守区域的其他基因产物。获得了与八个已知G蛋白α亚基中的六个相对应的序列。此外，我们还发现了与五个新发现的α亚基相对应的序列。我们的结果表明，G蛋白家族的复杂性比以前怀疑的要大得多。
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