Kang Heeyoung, Kim Haneul, Nam In-Young, Joung Yochan, Jang Tae Yong, Joh Kiseong
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, Republic of Korea.
Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2268-2273. doi: 10.1099/ijsem.0.001938. Epub 2017 Jul 12.
A novel Gram-stain-negative, aerobic and rod-shaped marine bacterium, designated strain HMF4135T, was isolated from a sand sample which was collected from the seashore of the South Sea, Republic of Korea. It required NaCl for growth and exhibited optimal growth at 30 °C, with 2 % (w/v) NaCl and at pH 7-8. Cellular fatty acids were dominated by C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω9c and C12 : 0 3-OH. The predominant isoprenoid quinone was ubiquinone-8 (Q-8). Polar lipids consisted of phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 41.9 mol%. Phylogeny based on 16S rRNA gene sequences showed that strain HMF4135T formed a distinct species-level lineage within the genus Thalassotalea of the class Gammaproteobacteria and was most closely related to Thalassotalea ponticola GJSW-36T (96.4 % similarity). Based on the distinctive phenotypic characteristics and phylogenetic analysis, it is concluded that strain HMF4135T represents a novel species of the genus Thalassotalea, for which the name Thalassotalea litorea sp. nov. is proposed. The type strain is HMF4135T (=KCTC 52154T=NBRC 112672T).
从韩国南海海滨采集的一份沙子样本中分离出了一种新型革兰氏阴性、需氧且呈杆状的海洋细菌，命名为菌株HMF4135T。其生长需要氯化钠，在30 °C、2 %（w/v）氯化钠及pH 7 - 8条件下生长最佳。细胞脂肪酸以C16 : 0、总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）、C16 : 1ω9c和C12 : 0 3 - OH为主。主要的类异戊二烯醌为泛醌-8（Q - 8）。极性脂类由磷脂酰甘油和磷脂酰乙醇胺组成。DNA的G + C含量为41.9 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株HMF4135T在γ-变形菌纲的海源杆菌属内形成了一个独特的物种水平分支，与海源杆菌Ponticola GJSW - 36T关系最为密切（相似性为96.4 %）。基于独特的表型特征和系统发育分析，得出结论：菌株HMF4135T代表海源杆菌属的一个新物种，为此提出名为海源滨海杆菌（Thalassotalea litorea sp. nov.）。模式菌株为HMF4135T（=KCTC 52154T=NBRC 112672T）。
