从嗜麦芽窄食单胞菌的多重耐药临床分离株中鉴定出一种新型6'-N-氨基糖苷乙酰转移酶AAC(6')-Iak。
Identification of a novel 6'-N-aminoglycoside acetyltransferase, AAC(6')-Iak, from a multidrug-resistant clinical isolate of Stenotrophomonas maltophilia.
作者信息
Tada Tatsuya, Miyoshi-Akiyama Tohru, Dahal Rajan K, Mishra Shyam K, Shimada Kayo, Ohara Hiroshi, Kirikae Teruo, Pokhrel Bharat M
机构信息
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan.
Department of Microbiology, Institute of Medicine, Tribhuvan University, Maharajgunj, Kathmandu, Nepal.
出版信息
Antimicrob Agents Chemother. 2014 Oct;58(10):6324-7. doi: 10.1128/AAC.03354-14. Epub 2014 Aug 4.
Abstract
Stenotrophomonas maltophilia IOMTU250 has a novel 6'-N-aminoglycoside acetyltransferase-encoding gene, aac(6')-Iak. The encoded protein, AAC(6')-Iak, consists of 153 amino acids and has 86.3% identity to AAC(6')-Iz. Escherichia coli transformed with a plasmid containing aac(6')-Iak exhibited decreased susceptibility to arbekacin, dibekacin, neomycin, netilmicin, sisomicin, and tobramycin. Thin-layer chromatography showed that AAC(6')-Iak acetylated amikacin, arbekacin, dibekacin, isepamicin, kanamycin, neomycin, netilmicin, sisomicin, and tobramycin but not apramycin, gentamicin, or lividomycin.
摘要
嗜麦芽窄食单胞菌IOMTU250具有一个新的编码6'-N-氨基糖苷乙酰转移酶的基因，aac(6')-Iak。所编码的蛋白质AAC(6')-Iak由153个氨基酸组成，与AAC(6')-Iz的同一性为86.3%。用含有aac(6')-Iak的质粒转化的大肠杆菌对阿贝卡星、地贝卡星、新霉素、奈替米星、西索米星和妥布霉素的敏感性降低。薄层色谱显示，AAC(6')-Iak可使阿米卡星、阿贝卡星、地贝卡星、异帕米星、卡那霉素、新霉素、奈替米星、西索米星和妥布霉素乙酰化，但不能使阿普拉霉素、庆大霉素或利维霉素乙酰化。
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