Wang Feng-Ying, Sun Yi-Ming, Zhang Hong, Cheng Meng-Qi, Zhang Lai, Sun Min
Zhong Yao Cai. 2014 Feb;37(2):179-82.
To study the condition of plant regeneration and clonal propagation system of Datura metel.
Stems and leaves of Datura metel were used as explants, effects of different hormones for callus induction and plant regeneration of leaves and clonal propagation system of stems were studied and optimized.
The optimal way to obtain sterile explant for leaves were sterilized in 75% ethyl alcohol for 6 s then 0.1% HgCl2 for 6 min; Stems were sterilized in 75% ethyl alcohol for 8 s then 0.1% HgCl2 for 7 min. The optimal medium for callus of leaves was MS + 1.0 mg/L 6-BA + 0.1 mg/L NAA; The optimal medium for callus induction of clustered buds was MS + 2.0 mg/L 6-BA + 0.2 mg/L NAA; The optimal medium for clonal propagation system of stems was MS + 3.0 mg/L 6-BA + 0.05 mg/L NAA. The best medium for rooting induction was MS + 0.5 mg/L IBA. Transplant survival rate of plantlet was greater than 90% in humus soil-pearlite (5:1).
The condition of plant regeneration and clonal propagation system of Datura metel is established.
研究白花曼陀罗的植株再生及克隆繁殖体系状况。
以白花曼陀罗的茎和叶为外植体，研究并优化不同激素对叶片愈伤组织诱导、植株再生及茎的克隆繁殖体系的影响。
叶片获得无菌外植体的最佳方法是在75%乙醇中消毒6 s，然后在0.1% HgCl₂中消毒6 min；茎在75%乙醇中消毒8 s，然后在0.1% HgCl₂中消毒7 min。叶片愈伤组织的最佳培养基为MS + 1.0 mg/L 6 - BA + 0.1 mg/L NAA；丛生芽愈伤组织诱导的最佳培养基为MS + 2.0 mg/L 6 - BA + 0.2 mg/L NAA；茎的克隆繁殖体系的最佳培养基为MS + 3.0 mg/L 6 - BA + 0.05 mg/L NAA。生根诱导的最佳培养基为MS + 0.5 mg/L IBA。在腐叶土 - 珍珠岩（5∶1）中，试管苗移栽成活率大于90%。
建立了白花曼陀罗的植株再生及克隆繁殖体系状况。
