Department of Bioscience and Biotechnology, Konkuk University , Seoul 143-701, Republic of Korea.
J Agric Food Chem. 2014 Aug 27;62(34):8764-71. doi: 10.1021/jf5020704. Epub 2014 Aug 14.
Metabolite profiling of red and white pitayas (Hylocereus polyrhizus and Hylocereus undatus) was performed using gas chromatography-time-of-flight-mass spectrometry and ultraperformance liquid chromatography-quadrupole-time-of-flight-mass spectrometry with multivariate analysis. Different species and parts of pitayas (red peel, RP; white peel, WP; red flesh, RF; and white flesh, WF) were clearly separated by partial least-squares discriminate analysis. Furthermore, betalain-related metabolites, such as betacyanins and betaxanthins, or their precursors were described on the basis of their metabolites. The results of antioxidant activity tests [1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and ferric reducing ability of plasma (FRAP)], total phenolic contents (TPC), total flavonoid contents (TFC), and total betacyanin contents (TBC) showed the following: RP ≥ WP > RF > WF. TPC, TFC, TBC, and betalain-related metabolites were higher in the peel than in the flesh and suggested to be the main contributors to antioxidant activity in pitayas. Therefore, peels as well as pulp of pitaya could beneficially help in the food industry.
采用气相色谱-飞行时间质谱联用技术和超高效液相色谱-四极杆飞行时间质谱联用技术，并结合多元分析方法，对火龙果（Hylocereus polyrhizus 和 Hylocereus undatus）的红色和白色品种进行代谢物轮廓分析。不同品种和火龙果部位（红色果皮、RP；白色果皮、WP；红色果肉、RF；白色果肉、WF）通过偏最小二乘判别分析可明显分离。此外，基于代谢产物描述了与甜菜红素相关的代谢物，如甜菜红素和甜菜黄素，或它们的前体。抗氧化活性测试[1,1-二苯基-2-苦基肼基（DPPH）、2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）和血浆铁还原能力（FRAP）]、总酚含量（TPC）、总黄酮含量（TFC）和总甜菜红素含量（TBC）的结果表明：RP≥WP>RF>WF。果皮中的 TPC、TFC、TBC 和甜菜红素相关代谢物均高于果肉，表明其是火龙果抗氧化活性的主要贡献者。因此，火龙果的果皮和果肉都有益于食品工业。
