Lopez-Huertas Eduardo, del Río Luis A
Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Estación Experimental del Zaidín (EEZ), Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, Granada 18008, Spain.
Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Estación Experimental del Zaidín (EEZ), Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, Granada 18008, Spain.
J Plant Physiol. 2014 Oct 15;171(16):1463-71. doi: 10.1016/j.jplph.2014.06.014. Epub 2014 Jul 8.
The presence of peroxisomes in olive (Olea europaea L.) fruits and different antioxidant enzymes occurring in this plant tissue is reported for the first time. Ultrastructural analysis showed that olive cells were characterized by the presence of large vacuoles and lipid drops. Plastids, mitochondria and peroxisomes were placed near the cell wall, showing some type of association with it. Olive fruit peroxisomes were purified by sucrose density-gradient centrifugation, and catalase, glutathione reductase and ascorbate peroxidase were found in peroxisomes. In olive fruit tissue the presence of a battery of antioxidant enzymes was demonstrated, including catalase, four superoxide dismutase isozymes (mainly an Fe-SOD plus 2 Cu,Zn-SOD and a Mn-SOD), all the enzymes of the ascorbate-glutathione cycle, reduced and oxidized glutathione, ascorbate, and four NADPH-recycling dehydrogenases. The knowledge of the full composition of antioxidants (enzymatic and non-enzymatic) in olive fruits is crucial to be able to understand the processes regulating the antioxidant composition of olive oil.
首次报道了橄榄（油橄榄）果实中过氧化物酶体的存在以及该植物组织中存在的不同抗氧化酶。超微结构分析表明，橄榄细胞的特征是存在大液泡和脂滴。质体、线粒体和过氧化物酶体位于细胞壁附近，显示出与细胞壁的某种关联。通过蔗糖密度梯度离心法纯化了橄榄果实过氧化物酶体，并在过氧化物酶体中发现了过氧化氢酶、谷胱甘肽还原酶和抗坏血酸过氧化物酶。在橄榄果实组织中，证实了一系列抗氧化酶的存在，包括过氧化氢酶、四种超氧化物歧化酶同工酶（主要是一种铁超氧化物歧化酶加两种铜锌超氧化物歧化酶和一种锰超氧化物歧化酶）、抗坏血酸-谷胱甘肽循环的所有酶、还原型和氧化型谷胱甘肽、抗坏血酸以及四种NADPH循环脱氢酶。了解橄榄果实中抗氧化剂（酶促和非酶促）的完整组成对于理解调节橄榄油抗氧化成分的过程至关重要。
