Planta Piloto de Procesos Industriales y Microbiológicos (PROIMI), CCT-CONICET, Av. Belgrano y Pasaje Caseros, 4000 Tucumán, Argentina.
Chemosphere. 2010 Mar;79(2):131-7. doi: 10.1016/j.chemosphere.2010.01.038. Epub 2010 Feb 16.
Amycolatopsis tucumanensis DSM 45259, the strain of a recently recognized novel species of the genus Amycolatopsis with remarkable copper resistance, was used to bioaugment soil microcosms experimentally polluted with copper and for studying the ability of this strain to effectively diminish phytoavailable copper from soils. Our results demonstrated that A. tucumanensis was capable of profusely colonizing both, copper polluted and non-polluted soil. Copper bioimmobilization ability of A. tucumanensis on soil was assessed measuring the bioavailable copper in the soil solution extracted from polluted soil by using chemical and physical methods and, in this way, 31% lower amounts of the metal were found in soil solution as compared to non-bioaugmented soil. The results obtained when using Zea mays as bioindicator correlated well with the values obtained by the chemical and physical procedures: 20% and 17% lower tissue contents of copper were measured in roots and leaves, respectively. These data confirmed the efficiency of the bioremediation process using A. tucumanensis and at the same time proved that chemical, physical and biological methods for assessing copper bioavailability in soils were correlated. These results suggest a potential use of this strain at large scale in copper soil bioremediation strategies. To our knowledge, this work is the first to apply and to probe the colonization ability of an Amycolatopsis strain in soil microcosms and constitutes the first application of an Amycolatopsis strain on bioremediation of polluted soils.
土拉弗朗西斯菌 DSM 45259，这是一个最近被确认的新型土拉弗朗西斯菌属菌株，具有显著的铜抗性，被用于生物强化实验污染的土壤微宇宙与铜和研究这种菌株有效地减少土壤中可利用的铜的能力。我们的结果表明，土拉弗朗西斯菌能够大量定殖在污染和非污染的土壤中。土拉弗朗西斯菌对土壤中铜的生物固定能力通过使用化学和物理方法测量从污染土壤中提取的土壤溶液中的可利用铜来评估，与未生物强化的土壤相比，土壤溶液中的金属含量低 31%。使用玉米作为生物指示剂得到的结果与化学和物理方法得到的结果很好地相关：在根和叶中分别测量到铜的组织含量低 20%和 17%。这些数据证实了使用土拉弗朗西斯菌进行生物修复过程的效率，同时证明了用于评估土壤中铜生物有效性的化学、物理和生物方法是相关的。这些结果表明，该菌株在大规模的铜污染土壤生物修复策略中有潜在的应用。据我们所知，这项工作首次应用并探测了土拉弗朗西斯菌菌株在土壤微宇宙中的定殖能力，并首次将土拉弗朗西斯菌菌株应用于污染土壤的生物修复。
