School of Biological Sciences, The University of Auckland, PO Box 92019, Auckland 1010, New Zealand.
School of Biological Sciences, The University of Auckland, PO Box 92019, Auckland 1010, New Zealand.
Virology. 2014 Sep;464-465:441-449. doi: 10.1016/j.virol.2014.07.005. Epub 2014 Aug 6.
A novel mycovirus tentatively assigned the name Sclerotinia sclerotiorum hypovirus 2 (SsHV2/5472) was detected in the phytopathogenic fungus Sclerotinia sclerotiorum. The genome is 14581 nucleotides (nts) long, excluding the poly (A) tail. A papain-like cysteine protease (Pro), an RNA-dependent RNA polymerase (RdRp) and a helicase (Hel) domain were detected in the polyprotein. Phylogenetic analysis based on multiple alignments of the aa sequence of the polyprotein placed it in a distinct clade from Alphahypovirus and Betahypovirus. The distinct aa sequence plus the fact that SsHV2/5472 possesses the longest reported genome for a hypovirus, suggests that SsHV2/5472 may represent a new genus in the family Hypoviridae. Eliminating SsHV2/5472 from S. sclerotiorum significantly increased the virulence of the protoplast virus-free derivative 5472-P5, although SsHV/5472-containing isolates showed significant variation in their virulence. In addition, membrane-bound vesicles (25-50 nm) were observed in ultrathin mycelial sections of SsHV2/5472 containing isolates but not in SsHV2/5472-free isolate.
一种暂定名为核盘菌潜病毒 2（SsHV2/5472）的新型真菌病毒在植物病原真菌核盘菌中被检测到。该基因组长 14581 个核苷酸（nt），不包括 poly（A）尾。在多蛋白中检测到木瓜蛋白酶样半胱氨酸蛋白酶（Pro）、RNA 依赖性 RNA 聚合酶（RdRp）和解旋酶（Hel）结构域。基于多蛋白 aa 序列的多重比对的系统发育分析将其置于与 Alphahypovirus 和 Betahypovirus 不同的分支中。独特的 aa 序列加上 SsHV2/5472 拥有报道的最短基因组，表明 SsHV2/5472 可能代表 Hypoviridae 家族中的一个新属。从核盘菌中消除 SsHV2/5472 显著增加了无质体病毒的原生质体衍生物 5472-P5 的毒力，尽管含有 SsHV/5472 的分离株在毒力上表现出显著的差异。此外，在含有 SsHV2/5472 的超薄菌丝切片中观察到膜结合囊泡（25-50nm），而在不含 SsHV2/5472 的分离株中则没有观察到。
