Xue Liang, Tao W Andy
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA ; Department of Medicinal Chemistry & Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA ; Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA ; Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.
Front Biol (Beijing). 2013 Apr 1;8(2):216-227. doi: 10.1007/s11515-013-1257-z.
Since the discovery of protein phosphorylation as an important modulator of many cellular processes, the involvement of protein kinases in diseases, such as cancer, diabetes, cardiovascular diseases, and central nervous system pathologies, has been extensively documented. Our understanding of many disease pathologies at the molecular level, therefore, requires the comprehensive identification of substrates targeted by protein kinases. In this review, we focus on recent techniques for kinase substrate identification in high throughput, in particular on genetic and proteomic approaches. Each method with its inherent advantages and limitations is discussed.
自从发现蛋白质磷酸化是许多细胞过程的重要调节因子以来，蛋白激酶在诸如癌症、糖尿病、心血管疾病和中枢神经系统疾病等疾病中的作用已被广泛记录。因此，我们在分子水平上对许多疾病病理的理解需要全面鉴定蛋白激酶的作用底物。在这篇综述中，我们重点关注高通量鉴定激酶底物的最新技术，特别是遗传学和蛋白质组学方法。我们将讨论每种方法的固有优缺点。
