橙皮苷对大鼠热缺血再灌注损伤中 Fas/FasL 凋亡途径表达的影响。
The effects of apigenin on the expression of Fas/FasL apoptotic pathway in warm liver ischemia-reperfusion injury in rats.
机构信息
Second Department of Surgery, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece ; Laboratory of Experimental Surgery and Surgical Research, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece.
Department of Pharmacology, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece.
出版信息
Biomed Res Int. 2014;2014:157216. doi: 10.1155/2014/157216. Epub 2014 Jul 6.
BACKGROUND
The aim of this experimental study was to investigate the role of apigenin in liver apoptosis, in an experimental model of hepatic ischemia-reperfusion in rats.
MATERIALS AND METHODS
Forty-eight Wistar rats (apigenin and control groups), 14 to 16 weeks old and weighing 220 to 350 g, were used. They were all subjected to hepatic ischemia by occlusion of the hepatic artery and portal vein for 45 minutes and reperfusion was followed for 60, 120, and 240 minutes. Apigenin was administrated intraperitoneally. Liver tissues were used for the detection of apoptosis by TUNEL assay and caspase 3 antibodies. Expression analysis of Fas/FasL genes was evaluated by real time PCR.
RESULTS
The expression analysis of Fas and FasL genes was increasing during reperfusion (significantly in the group of 240 minutes of reperfusion). It was in the same group that apigenin decreased Fas receptor levels and inhibited apoptosis as confirmed by TUNEL assay and caspase 3 antibodies.
CONCLUSIONS
The effects of apigenin in the Fas/FasL mediated pathway of apoptosis, in the hepatic ischemia-reperfusion, seem to have a protective result on the hepatic cell.
背景
本实验研究旨在探讨芹菜素在肝缺血再灌注大鼠模型中肝细胞凋亡中的作用。
材料与方法
将 48 只 14-16 周龄、体重 220-350g 的 Wistar 大鼠（芹菜素组和对照组）用于本实验。通过阻断肝动脉和门静脉使肝脏缺血 45 分钟，然后再灌注 60、120 和 240 分钟。采用腹腔内注射给予芹菜素。采用 TUNEL 检测和 caspase 3 抗体检测肝组织细胞凋亡，实时 PCR 检测 Fas/FasL 基因表达分析。
结果
在再灌注过程中 Fas 和 FasL 基因的表达分析增加（在再灌注 240 分钟组中显著增加）。正是在该组中，芹菜素降低了 Fas 受体水平，并通过 TUNEL 检测和 caspase 3 抗体证实抑制了细胞凋亡。
结论
在肝缺血再灌注中，芹菜素在 Fas/FasL 介导的细胞凋亡途径中的作用似乎对肝细胞具有保护作用。
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