三价和六价铬化合物的体内遗传毒性及其体外对DNA损伤的作用模式。
Genotoxicity of tri- and hexavalent chromium compounds in vivo and their modes of action on DNA damage in vitro.
作者信息
Fang Zhijia, Zhao Min, Zhen Hong, Chen Lifeng, Shi Ping, Huang Zhiwei
机构信息
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
出版信息
PLoS One. 2014 Aug 11;9(8):e103194. doi: 10.1371/journal.pone.0103194. eCollection 2014.
Chromium occurs mostly in tri- and hexavalent states in the environment. Hexavalent chromium [Cr(VI)] compounds are extensively used in diverse industries, and trivalent chromium [Cr(III)] salts are used as micronutrients and dietary supplements. In the present work, we report that they both induce genetic mutations in yeast cells. They both also cause DNA damage in both yeast and Jurkat cells and the effect of Cr(III) is greater than that of Cr(VI). We further show that Cr(III) and Cr(VI) cause DNA damage through different mechanisms. Cr(VI) intercalates DNA and Cr(III) interferes base pair stacking. Based on our results, we conclude that Cr(III) can directly cause genotoxicity in vivo.
铬在环境中大多以三价和六价状态存在。六价铬[Cr(VI)]化合物广泛应用于各种行业，而三价铬[Cr(III)]盐用作微量营养素和膳食补充剂。在本研究中，我们报告它们都能在酵母细胞中诱导基因突变。它们还都会在酵母细胞和Jurkat细胞中造成DNA损伤，且Cr(III)的影响大于Cr(VI)。我们进一步表明，Cr(III)和Cr(VI)通过不同机制造成DNA损伤。Cr(VI)嵌入DNA，而Cr(III)干扰碱基对堆积。基于我们的结果，我们得出结论，Cr(III)可在体内直接引起遗传毒性。
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