Chhibber Sanjay, Gupta Paridhi, Kaur Sandeep
BMC Microbiol. 2014 Aug 12;14:212. doi: 10.1186/s12866-014-0212-8.
Nasal carriers not only pose serious threat to themselves but also to the community by playing an active role in the dissemination of serious and life threatening S. aureus especially MRSA strains. The present study focuses on the use of broad spectrum lytic phage as decolonising agent. In addition, the combined use of lytic phage with mupirocin has also been investigated as an effective decolonising regimen. The effect of phage on the adherence, invasion and cytotoxic effect of MRSA strains on nasal epithelial cells was studied in an ex-vivo model of cultured murine nasal epithelial cells. This was followed by demonstration of therapeutic potential of phage along with mupirocin in decolonising the nares of BALB/c mice using a nasal model of MRSA colonisation.
Phage was able to significantly reduce the in vitro adherence, invasion and cytotoxicity of MRSA 43300 as well as other clinical MRSA strains on murine nasal epithelial cells as compared to untreated control. Also, the frequency of emergence of spontaneous mutants decreased to negligible levels when both the agents (phage and mupirocin) were used together.
Phage MR-10, given along with mupirocin showed an additive effect and the combination was able to effectively eradicate the colonising MRSA population from the nares of mice by day 5.
鼻腔携带者不仅对自身构成严重威胁，还通过在传播严重且危及生命的金黄色葡萄球菌（尤其是耐甲氧西林金黄色葡萄球菌菌株）中发挥积极作用，对社区构成威胁。本研究重点关注使用广谱裂解噬菌体作为去定植剂。此外，还研究了裂解噬菌体与莫匹罗星联合使用作为一种有效的去定植方案。在培养的小鼠鼻上皮细胞的体外模型中，研究了噬菌体对耐甲氧西林金黄色葡萄球菌菌株在鼻上皮细胞上的黏附、侵袭和细胞毒性作用的影响。随后，使用耐甲氧西林金黄色葡萄球菌定植的鼻腔模型，证明了噬菌体与莫匹罗星在使BALB/c小鼠鼻腔去定植方面的治疗潜力。
与未处理的对照相比，噬菌体能够显著降低耐甲氧西林金黄色葡萄球菌43300以及其他临床耐甲氧西林金黄色葡萄球菌菌株在小鼠鼻上皮细胞上的体外黏附、侵袭和细胞毒性。此外，当同时使用两种药物（噬菌体和莫匹罗星）时，自发突变体的出现频率降低到可忽略不计的水平。
噬菌体MR-10与莫匹罗星联合使用显示出相加作用，并且该组合能够在第5天时有效从小鼠鼻腔根除定植的耐甲氧西林金黄色葡萄球菌群体。
