Grönroos J O, Laine V J, Janssen M J, Egmond M R, Nevalainen T J
Department of Pathology, University of Turku and Turku University Hospital, Turku, Finland.
J Immunol. 2001 Mar 15;166(6):4029-34. doi: 10.4049/jimmunol.166.6.4029.
Group V phospholipase A(2) (PLA(2)) is a recently characterized 14-kDa secretory PLA(2) of mammalian heart and macrophage-derived cells. Group IIA PLA(2), which is structurally close to group V PLA(2), has been shown to kill Gram-positive bacteria in vitro and to prevent symptoms of Gram-positive infection in vivo. We studied the antibacterial properties of fully active recombinant rat group IIA and V PLA(2)s. Both group IIA and V PLA(2)s were highly bactericidal against Gram-positive bacteria, including methicillin-resistant staphylococci and vancomycin-resistant enterococci. Only high concentrations of group IIA PLA(2) showed some bactericidal effect against the Gram-negative bacterium Escherichia coli. Our results confirm that group IIA PLA(2) is a potent antibacterial enzyme against Gram-positive bacteria. Moreover, we show here that group V PLA(2) is a novel antibacterial mammalian protein, but is less potent than group IIA PLA(2). Both enzymes may be considered as future therapeutic agents against bacterial infections.
V组磷脂酶A2（PLA2）是一种最近鉴定出的分子量为14 kDa的哺乳动物心脏和巨噬细胞衍生细胞分泌型PLA2。结构上与V组PLA2接近的IIA组PLA2已被证明在体外可杀死革兰氏阳性菌，并在体内预防革兰氏阳性菌感染症状。我们研究了完全活性的重组大鼠IIA组和V组PLA2的抗菌特性。IIA组和V组PLA2对革兰氏阳性菌均具有高度杀菌活性，包括耐甲氧西林葡萄球菌和耐万古霉素肠球菌。只有高浓度的IIA组PLA2对革兰氏阴性菌大肠杆菌表现出一定的杀菌作用。我们的结果证实，IIA组PLA2是一种针对革兰氏阳性菌的强效抗菌酶。此外，我们在此表明，V组PLA2是一种新型的哺乳动物抗菌蛋白，但效力低于IIA组PLA2。这两种酶都可被视为未来抗细菌感染的治疗药物。
