Iroanya Onyekachi Ogbonnaya, Adebesin Olumide Adedapo, Okpuzor Joy
Lecturer, Department of Cell Biology and Genetics, University of Lagos , Akoka - Yaba, Lagos, Nigeria .
Assistant Lecturer, Department of Cell Biology and Genetics, University of Lagos , Akoka - Yaba, Lagos, Nigeria .
J Clin Diagn Res. 2014 Jun;8(6):HC15-21. doi: 10.7860/JCDR/2014/5875.4501. Epub 2014 Jun 20.
A polyherbal formulation prepared from a mixture of leaves of Gongronema latifolia, Ocimum gratissimum and Vernonia amygdalina (GOV) was evaluated for hepato-nephro protective properties against acetaminophen-induced toxicity in Wistar albino rats.
Normal Wistar albino rats were orally treated with different doses of GOV extract (2, 4 and 8 g/kg b. wt), distilled water and some standard hepatoprotective drugs such as Liv 52 and silymarin for 14 days. However, a day prior to the 14th day, 3 g/kg body weight dose of Acetaminophen (APAP) was administered p.o. 1h before GOV and the standard drugs to induce hepatic and renal damage. The normal control was setup which received only distilled water. The serum levels of liver marker enzymes, biochemical analytes, antioxidant enzymes and hematological parameters were monitored.
The results showed that pretreatment of experimental animals with a different doses of the polyherbal formulation dose dependently caused a significant (p≤0.05) increase in the levels of most of the measured hematological parameters but significantly (p≤0.05) reduced the levels of MCV and monocytes when compared to the APAP induced toxin control group. Rats pretreated with GOV exhibited significant (p < 0.05) increase in serum levels of ALP, ALT, AST, GGT, LDH, Cholesterol, Triglycerides, Urea and a subsequent decrease in Albumin, Creatine and Total protein when compared to the normal rats. This trend in enzyme and biochemical analytes levels were significantly (p < 0.05) reversed when compared to toxin control group. GOV significantly (p < 0.05) and dose dependently increased the serum, kidney and hepatic CAT, GPx, GSH, GST, SOD and total protein activity in APAP induced damage in rats compared to the toxin control groups.
The data from this study suggest that the polyherbal formulation possess hepato and nephron-protective potential against acetaminophen induced hepatotoxicity in rats, thus providing scientific rationale for its use in traditional medicine for the treatment of liver diseases.
评估由宽叶锣麻、丁香罗勒和扁桃斑鸠菊（GOV）叶混合物制备的多草药配方对乙酰氨基酚诱导的Wistar白化大鼠肝肾毒性的保护作用。
将正常Wistar白化大鼠口服不同剂量的GOV提取物（2、4和8 g/kg体重）、蒸馏水以及一些标准保肝药物如利肝隆和水飞蓟宾，持续14天。然而，在第14天前一天，在给予GOV和标准药物前1小时口服3 g/kg体重剂量的对乙酰氨基酚（APAP）以诱导肝和肾损伤。设立仅接受蒸馏水的正常对照组。监测血清肝标志物酶、生化分析物、抗氧化酶和血液学参数水平。
结果表明，与APAP诱导的毒素对照组相比，用不同剂量的多草药配方预处理实验动物，大多数测定的血液学参数水平呈剂量依赖性显著（p≤0.05）升高，但MCV和单核细胞水平显著（p≤0.05）降低。与正常大鼠相比，用GOV预处理的大鼠血清碱性磷酸酶、谷丙转氨酶、谷草转氨酶、γ-谷氨酰转肽酶、乳酸脱氢酶、胆固醇、甘油三酯、尿素水平显著（p < 0.05）升高，随后白蛋白、肌酸和总蛋白水平降低。与毒素对照组相比，酶和生化分析物水平的这种趋势显著（p < 0.05）逆转。与毒素对照组相比，GOV显著（p < 0.05）且剂量依赖性地增加了APAP诱导的大鼠损伤中的血清、肾脏和肝脏过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽、谷胱甘肽S-转移酶、超氧化物歧化酶和总蛋白活性。
本研究数据表明，该多草药配方对大鼠对乙酰氨基酚诱导的肝毒性具有肝和肾保护潜力，从而为其在传统医学中用于治疗肝脏疾病提供了科学依据。
