Max-Planck-Institut für Biochemie, D-8033 Martinsried bei München, FRG.
EMBO J. 1982;1(9):1035-8. doi: 10.1002/j.1460-2075.1982.tb01292.x.
A plasmid of mol. wt. approximately 9 x 10 has been isolated from the archaebacterium Sulfolobus acidocaldarius strain B12. Plasmid production is induced by u.v. radiation. A copy of the plasmid is probably carried by the chromosome, integrated at a specific site. The entire plasmid, and also restriction fragments of it, has been cloned into Escherichia coli plasmid vectors, and the cleavage sites on the plasmid DNA of three restriction endonucleases have been mapped.
已从古细菌嗜酸热硫化叶菌 B12 株中分离出分子量约为 9×10 的质粒。紫外线辐射可诱导质粒产生。质粒的一个拷贝可能存在于染色体中,并整合在特定的位置上。整个质粒,以及它的限制片段,已被克隆到大肠杆菌质粒载体中,并对三种限制内切酶的质粒 DNA 的切割位点进行了定位。