桑白皮的化学成分及其对3T3-L1前脂肪细胞增殖和分化的抑制作用。

Chemical constituents of Morus alba L. and their inhibitory effect on 3T3-L1 preadipocyte proliferation and differentiation.

作者信息

Yang Yongyu, Yang Xiding, Xu Bing, Zeng Guangyao, Tan Jianbing, He Xi, Hu Changping, Zhou YingJun

机构信息

Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China; Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.

Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.

出版信息

Fitoterapia. 2014 Oct;98:222-7. doi: 10.1016/j.fitote.2014.08.010. Epub 2014 Aug 12.

DOI:10.1016/j.fitote.2014.08.010

PMID:25128426

Abstract

Mulberry leaf, an important traditional Chinese medicine, possesses many biological activities, including effects of anti-obesity. However, which constituents of mulberry leaf are responsible for its anti-adipogenic action is unclear. This study primarily investigated the chemical constituents from mulberry leaf and their bioactivity on the proliferation and differentiation of 3T3-L1 preadipocytes. A new flavane derivative, (2S)-4'-hydroxy-7-methoxy-8-prenylflavan (1), together with twelve known compounds including three flavanes (2-4), three chalcones (5-7), two flavones (8-9), two benzofurans (10-11) and two coumarin (12-13) was isolated from mulberry leaf. The structure of the new compound was elucidated by various spectroscopic methods including UV, HR-ESI-MS, (1)H and (13)C NMR and CD. The results of activity screening showed that compound 2, 6 and 7 inhibited the proliferation and differentiation of 3T3-L1 preadipocytes.

摘要

桑叶是一种重要的传统中药，具有多种生物活性，包括抗肥胖作用。然而，桑叶中哪些成分具有抗脂肪生成作用尚不清楚。本研究主要研究了桑叶的化学成分及其对3T3-L1前脂肪细胞增殖和分化的生物活性。从桑叶中分离出一种新的黄烷衍生物，(2S)-4'-羟基-7-甲氧基-8-异戊烯基黄烷(1)，以及12种已知化合物，包括三种黄烷(2-4)、三种查耳酮(5-7)、两种黄酮(8-9)、两种苯并呋喃(10-11)和两种香豆素(12-13)。通过紫外光谱、高分辨电喷雾电离质谱、氢核磁共振和碳核磁共振以及圆二色光谱等多种光谱方法对新化合物的结构进行了鉴定。活性筛选结果表明，化合物2、6和7抑制3T3-L1前脂肪细胞的增殖和分化。