Bio-Center, Gyeonggido Business & Science Accelerator, Suwon 16229, Republic of Korea.
College of Pharmacy, Dankook University, Cheonan 31116, Republic of Korea.
Int J Mol Sci. 2023 May 16;24(10):8866. doi: 10.3390/ijms24108866.
, a climbing tree belonging to Smilacaceae, has been used in traditional oriental medicine for treating arthritis, tumors, leprosy, psoriasis, and lumbago. To evaluate the anti-obesity effects of (Smilacaceae), we screened methylene chloride (CHCl), ethyl acetate (EtOAc), aqueous-saturated -butanol, and ethanol (EtOH) extracts of the whole plant at various concentrations to inhibit adipogenesis in adipocytes. The 3T3-L1 cell line with Oil red O staining with the help of fluorometry was used as an indicator of anti-obesity activity. Bioactivity-guided fractionation of the EtOH extract and subsequent phytochemical investigation of the active CHCl- and EtOAc-soluble fractions resulted in the isolation of 19 secondary metabolites (-), including a new α-hydroxy acid derivative () and two new lanostane-type triterpenoids ( and ). The structures of these compounds were characterized using various spectroscopic methods. All the isolated compounds were screened for adipogenesis inhibition at a concentration of 100 μM. Of these, compounds , , -, , and significantly reduced fat accumulation in 3T3-L1 adipocytes, especially compounds , , , and , showing 37.05 ± 0.95, 8.60 ± 0.41 15.82 ± 1.23, and 17.73 ± 1.28% lipid content, respectively, at a concentration of 100 μM. These findings provide experimental evidence that isolates from extracts exert beneficial effects regarding the regulation of adipocyte differentiation.
菝葜,属于百合科攀援植物,在传统的东方医学中用于治疗关节炎、肿瘤、麻风病、银屑病和腰痛。为了评估菝葜(百合科)的抗肥胖作用,我们在不同浓度下筛选了整株植物的二氯甲烷(CHCl)、乙酸乙酯(EtOAc)、水饱和正丁醇和乙醇(EtOH)提取物,以抑制脂肪细胞中的脂肪生成。使用荧光法进行油红 O 染色的 3T3-L1 细胞系被用作抗肥胖活性的指示剂。乙醇提取物的生物活性导向分离以及随后对活性 CHCl 和 EtOAc 可溶部分的植物化学研究导致分离出 19 种次生代谢产物(-),包括一种新的α-羟基酸衍生物()和两种新的甾体三萜(和)。这些化合物的结构通过各种光谱方法进行了表征。所有分离出的化合物都在 100 μM 浓度下进行了脂肪生成抑制筛选。其中,化合物、、、、和显著抑制了 3T3-L1 脂肪细胞中的脂肪积累,特别是化合物、、、和,在 100 μM 浓度下分别显示出 37.05±0.95%、8.60±0.41%、15.82±1.23%和 17.73±1.28%的脂质含量。这些发现为来自提取物的分离物在调节脂肪细胞分化方面具有有益作用提供了实验证据。