Doğan Ayşegül, Demirci Selami, Cağlayan Ahmet B, Kılıç Ertuğrul, Günal Mehmet Y, Uslu Unal, Cumbul Alev, Sahin Fikrettin
Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University Kayisdagi, Istanbul, Turkey, 34755.
Biol Trace Elem Res. 2014 Dec;162(1-3):72-9. doi: 10.1007/s12011-014-0104-7. Epub 2014 Aug 17.
After a disruption of skin integrity, the body produces an immediate response followed by a functional and comparable regeneration period, referred to as wound healing. Although normal wounds do not need much attention during the healing period, chronic (non-healing) wounds are the major challenge of current dermatological applications. Therefore, developing new, safe, and effective wound healing drugs has always been an attractive area of international research. In the current study, sodium pentaborate pentahydrate (NaB), pluronics (Plu; F68 and F127), and their combinations were investigated for their wound healing activities, using in vitro and in vivo approaches. The results revealed that NaB significantly increased migration capacity and superoxide dismutase activity in primary human fibroblasts. Combinations of optimized concentrations for pluronic block co-polymers further increased cell migration, and the messenger RNA (mRNA) expression levels of important growth factor and cytokines (vascular endothelial growth factor (VEGF), transforming growth factor beta (TGF-β), and tumor necrosis factor alpha (TNF-α)). NaB containing hydrogel co-formulated with pluronics was also investigated for their wound healing activities using a full thickness wound model in rats. Macroscopic and histopathological analysis confirmed that wounds in combination gel-treated groups healed faster than those of control groups. NaB/Plu gel application was found to increase wound contraction and collagen deposition in the wound area. Therefore, our results suggest that NaB, and its pluronics combination, could be used in dermatological clinics and be a future solution for chronic wounds. However, further studies should be conducted to explore its exact action of mechanism and effects of this formulation on chronic wounds.
皮肤完整性遭到破坏后，机体立即产生反应，随后进入功能上类似的再生期，即伤口愈合。虽然正常伤口在愈合期间无需过多关注，但慢性（不愈合）伤口是当前皮肤病学应用中的主要挑战。因此，开发新型、安全且有效的伤口愈合药物一直是国际研究的一个热门领域。在本研究中，采用体外和体内方法，对五水硼酸钠（NaB）、普朗尼克（Plu；F68和F127）及其组合的伤口愈合活性进行了研究。结果显示，NaB显著提高了原代人成纤维细胞的迁移能力和超氧化物歧化酶活性。普朗尼克嵌段共聚物优化浓度的组合进一步提高了细胞迁移以及重要生长因子和细胞因子（血管内皮生长因子（VEGF）、转化生长因子β（TGF-β）和肿瘤坏死因子α（TNF-α））的信使核糖核酸（mRNA）表达水平。还使用大鼠全层伤口模型研究了与普朗尼克共配制的含NaB水凝胶的伤口愈合活性。宏观和组织病理学分析证实，联合凝胶治疗组的伤口比对照组愈合得更快。发现应用NaB/Plu凝胶可增加伤口收缩和伤口区域的胶原沉积。因此，我们的结果表明，NaB及其与普朗尼克的组合可用于皮肤科诊所，可能是治疗慢性伤口的未来解决方案。然而，应进一步开展研究以探索其确切作用机制以及该制剂对慢性伤口的影响。
