Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Kayısdagi Cad., Atasehir, 34755, Istanbul, Turkey.
Department of Molecular Biology and Genetics, Faculty of Natural Sciences, Istanbul University, 34134, Istanbul, Turkey.
Sci Rep. 2023 Sep 1;13(1):14404. doi: 10.1038/s41598-023-40809-y.
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death in the world. Poor prognosis of HCC patients is a major issue, thus, better treatment options for patients are required. Curcumin (Cur), hydrophobic polyphenol of the plant turmeric, shows anti-proliferative, apoptotic, and anti-oxidative properties. Boron is a trace element which is essential part of human nutrition. Sodium pentaborate pentahydrate (NaB), a boron derivative, is an effective agent against cancer. In the current study, we performed in vitro experiments and transcriptome analysis to determine the response of NaB, Cur, piperine (Pip) and their combination in two different HCC cell lines, HepG2 and Hep3B. NaB and Cur induced cytotoxicity in a dose and time dependent manner in HepG2 and Hep3B, whereas Pip showed no significant toxic effect. Synergistic effect of combined treatment with NaB, Cur and Pip on HCC cells was observed on cytotoxicity, apoptosis and cell cycle assay. Following in vitro studies, we performed RNA-seq transcriptome analysis on NaB, Cur and Pip and their combination on HepG2 and Hep3B cells. Transcriptome analysis reveals combined treatment of NaB, Cur and Pip induces anti-cancer activity in both of HCC cells.
肝细胞癌 (HCC) 是全球癌症相关死亡的主要原因。HCC 患者的预后较差，因此需要为患者提供更好的治疗选择。姜黄素 (Cur) 是植物姜黄中的一种疏水性多酚，具有抗增殖、凋亡和抗氧化作用。硼是一种微量元素，是人类营养的重要组成部分。五硼酸钠五水合物 (NaB) 是一种硼衍生物，是一种有效的抗癌药物。在本研究中，我们进行了体外实验和转录组分析，以确定 NaB、Cur、胡椒碱 (Pip) 及其组合在两种不同的 HCC 细胞系 HepG2 和 Hep3B 中的反应。NaB 和 Cur 以剂量和时间依赖的方式诱导 HepG2 和 Hep3B 细胞的细胞毒性，而 Pip 则没有显示出明显的毒性作用。NaB、Cur 和 Pip 联合治疗对 HCC 细胞的细胞毒性、细胞凋亡和细胞周期检测显示出协同作用。在体外研究之后，我们对 NaB、Cur 和 Pip 及其组合对 HepG2 和 Hep3B 细胞的 RNA-seq 转录组进行了分析。转录组分析表明，NaB、Cur 和 Pip 的联合治疗在两种 HCC 细胞中均诱导了抗癌活性。
