不同硼化合物对养分消化率、肠道养分转运体和肝脏脂质代谢的影响。
The effect of different boron compounds on nutrient digestibility, intestinal nutrient transporters, and liver lipid metabolism.
机构信息
Department of Animal Nutrition, Faculty of Veterinary Medicine, Bingöl University, Bingöl, Turkey.
Department of Animal Nutrition, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
出版信息
Turk J Med Sci. 2023 Jun;53(3):619-629. doi: 10.55730/1300-0144.5624. Epub 2023 Jun 19.
BACKGROUND
Gastrointestinal health is essential for maintaining a healthy lifestyle. Improving nutrient absorption and energy metabolism are the critical targets for intestinal health. This study aimed to determine the effects of different boron (B) derivatives on nutrient digestibility, intestinal nutrient transporters, and lipid metabolism in rats.
METHODS
Twenty-one rats were allocated to three groups (n = 7) as follows: (i) Control, (ii) Sodium pentaborate pentahydrate (SPP), and (iii) boric acid (BA). The rats were fed a chow diet (AIN-93M) and supplemented with 8 mg/kg elemental B from SPP (45.2 mg/kg BW) and BA (42.7 mg/kg BW) via oral gavage every other day for 12 weeks. The nutrient digestibility of rats in each group was measured using the indigestible indicator (chromium oxide, Cr2 O3, 0.20%). At the end of the experiment, animals were decapitated by cervical dislocation and jejunum, and liver samples were taken from each animal. The nutrient transporters and lipid-regulated transcription factors were determined by RT-PCR.
RESULTS
The nutrient digestibility (except for ash) was increased by SPP and BA supplementation (p < 0.05). SPP and BA-supplemented rats had higher jejunal glucose transporter 1 (GLUT1), GLUT2, GLUT5, sodium-dependent glucose transporter 1 (SGLT1), fatty acid transport protein-1 (FATP1), and FATP4 mRNA expression levels compared to nonsupplemented rats (p < 0.0001). BA-supplemented rats had remarkably higher peroxisome proliferator-activated receptor gamma (PPARγ) levels than nonsupplemented rats (p < 0.0001). In contrast, sterol regulatory element-binding protein 1c (SREBP-1c), liver X receptor alpha (LxR-α), and fatty acid synthase (FAS) levels decreased by SPP supplementation compared to other groups (p < 0.05).
DISCUSSION
SPP and BA administration enhanced nutrient digestibility, intestinal nutrient transporters, and liver lipid metabolism in rats.
背景
胃肠道健康对于维持健康的生活方式至关重要。改善营养吸收和能量代谢是肠道健康的关键目标。本研究旨在确定不同硼（B）衍生物对大鼠营养消化率、肠道营养转运体和脂质代谢的影响。
方法
将 21 只大鼠分为三组（n = 7）：（i）对照组、（ii）五硼酸钠五水合物（SPP）组和（iii）硼酸（BA）组。大鼠喂食AIN-93M 标准饲料，并通过口服灌胃每天补充 8mg/kg 元素硼，分别来自 SPP（45.2mg/kgBW）和 BA（42.7mg/kgBW），每隔一天一次，持续 12 周。每组大鼠的营养消化率通过不消化指示剂（氧化铬，Cr2O3，0.20%）进行测量。实验结束时，通过颈椎脱位处死动物，并从每个动物中取出空肠和肝脏样本。通过 RT-PCR 测定营养转运体和脂质调节转录因子。
结果
SPP 和 BA 补充均增加了营养消化率（除灰分外）（p<0.05）。与未补充组相比，SPP 和 BA 补充组大鼠空肠葡萄糖转运蛋白 1（GLUT1）、GLUT2、GLUT5、钠依赖性葡萄糖转运蛋白 1（SGLT1）、脂肪酸转运蛋白-1（FATP1）和 FATP4mRNA 表达水平更高（p<0.0001）。与未补充组相比，BA 补充组大鼠过氧化物酶体增殖物激活受体γ（PPARγ）水平显著更高（p<0.0001）。相反，与其他组相比，SPP 补充组大鼠固醇调节元件结合蛋白 1c（SREBP-1c）、肝 X 受体α（LxR-α）和脂肪酸合成酶（FAS）水平降低（p<0.05）。
讨论
SPP 和 BA 给药增强了大鼠的营养消化率、肠道营养转运体和肝脏脂质代谢。
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