de Rond Tristan, Danielewicz Megan, Northen Trent
Dept. of Chemistry, University of California, Berkeley, CA 94720, USA.
Lawrence Berkeley National Lab, One Cyclotron Road, Berkeley, CA 94720, USA.
Curr Opin Biotechnol. 2015 Feb;31:1-9. doi: 10.1016/j.copbio.2014.07.008. Epub 2014 Aug 15.
Mass spectrometry imaging (MSI) has found a diversity of applications ranging from localizing metabolites and proteins in tissues to investigating microbial interactions, and as a result is perhaps the fastest growing subfield of mass spectrometry. Advances in surface mass spectrometry technologies are equally applicable to the analysis of arrayed samples. One promising field in which this capacity has been leveraged is the high-throughput analysis of enzyme activity, an important step in the development of a wide range of biotechnologies. This review article describes several emerging approaches that seek to improve the quality and scope of this application of MSI.
质谱成像（MSI）已发现了多种多样的应用，从在组织中定位代谢物和蛋白质到研究微生物相互作用，因此它可能是质谱领域中发展最快的子领域。表面质谱技术的进展同样适用于阵列样品的分析。利用这种能力的一个有前景的领域是酶活性的高通量分析，这是广泛生物技术开发中的一个重要步骤。这篇综述文章描述了几种旨在提高MSI这种应用的质量和范围的新兴方法。
