Silva Leslie P, Northen Trent R
Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA; Joint Genome Institute, Department of Energy, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA; Joint Genome Institute, Department of Energy, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
Curr Opin Biotechnol. 2015 Aug;34:209-16. doi: 10.1016/j.copbio.2015.03.015. Epub 2015 Apr 5.
Metabolism is at the heart of many biotechnologies from biofuels to medical diagnostics. Metabolomic methods that provide glimpses into cellular metabolism have rapidly developed into a critical component of the biotechnological development process. Most metabolomics methods have focused on what is happening inside the cell. Equally important are the biochemical transformations of the cell, and their effect on other cells and their environment; the exometabolome. Exometabolomics is therefore gaining popularity as a robust approach for obtaining rich phenotypic data, and being used in bioprocessing and biofuel development. Mass spectrometry imaging approaches, including several nanotechnologies, provide complimentary information by localizing metabolic processes within complex biological matrices. Together, the two technologies can provide new insights into the metabolism and interactions of cells.
代谢是从生物燃料到医学诊断等众多生物技术的核心。能够洞察细胞代谢的代谢组学方法已迅速发展成为生物技术开发过程的关键组成部分。大多数代谢组学方法都聚焦于细胞内部正在发生的事情。细胞的生化转化及其对其他细胞及其环境的影响，即胞外代谢组，同样重要。因此，胞外代谢组学作为一种获取丰富表型数据的可靠方法正日益受到欢迎，并被应用于生物加工和生物燃料开发。包括几种纳米技术在内的质谱成像方法，通过在复杂生物基质中定位代谢过程来提供补充信息。这两种技术结合起来，可以为细胞的代谢和相互作用提供新的见解。
