博尔氏果实多糖的纯化、结构与抗氧化性
Purification, structure and anti-oxidation of polysaccharides from the fruit of Bobr.
作者信息
Zhao Baotang, Liu Jing, Chen Xin, Zhang Ji, Wang Junlong
机构信息
College of Food Science and Engineering, Gansu Agricultural University Lanzhou 730070 China
Bioactive Products Engineering Research Center for Gansu Distinctive Plants, Northwest Normal University Lanzhou 730070 China.
出版信息
RSC Adv. 2018 Mar 27;8(21):11731-11743. doi: 10.1039/c8ra01125g. eCollection 2018 Mar 21.
In this paper, polysaccharides were extracted from the fruits of Bobr. (NTWP) using a hot water extraction method and extraction conditions were optimized by RSM. The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%. After purification using DEAE-cellulose column and Sephadex G-200 column, NTWP-II was successfully obtained. The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and , / and 2.29 × 10, 1.32, 15.22. The detailed structure of NTWP-II was characterized by FT-IR, NMR. Based on these analyses, the structure of the repeating unit of NTWP-II was established.
本文采用热水提取法从Bobr.（NTWP）果实中提取多糖，并通过响应面法（RSM）优化提取条件。确定的最佳条件如下：提取时间7小时，提取温度60℃，料液比15∶1，在此条件下，得率为14.01±0.11%。经DEAE-纤维素柱和Sephadex G-200柱纯化后，成功获得NTWP-II。气相色谱-质谱联用（GC-MS）和体积排阻色谱-多角度激光光散射（SEC-LLS）分析结果表明，NTWP-II的单糖组成由鼠李糖（Rha）、阿拉伯糖（Ara）、甘露糖（Man）、葡萄糖（Glc）和半乳糖（Gal）组成，摩尔比为1.14∶2.5∶3.00∶2.69∶5.28，重均分子量（Mw）/数均分子量（Mn）和Mw分别为2.29×10、1.32、15.22。通过傅里叶变换红外光谱（FT-IR）、核磁共振（NMR）对NTWP-II的详细结构进行了表征。基于这些分析，确定了NTWP-II重复单元的结构。
Zotero 插件