使用单克隆抗体检测细胞培养支原体的快速简便方法。

Fast and simple procedure for the detection of cell culture mycoplasmas using a single monoclonal antibody.

作者信息

Blazek R, Schmitt K, Krafft U, Hadding U

机构信息

Institute for Medical Microbiology and Virology, Heinrich-Heine-University, Düsseldorf, F.R.G.

出版信息

J Immunol Methods. 1990 Aug 7;131(2):203-12. doi: 10.1016/0022-1759(90)90191-w.

DOI:10.1016/0022-1759(90)90191-w

PMID:1697315

Abstract

The detection of mycoplasmas in cell cultures is still a problem, especially in those laboratories in which the detection and identification of microorganisms is not established as a routine procedure. In our laboratory, monoclonal antibodies (mAbs) have been prepared to Acholeplasma laidlawii, Mycoplasma hyorhinis, Mycoplasma orale, Mycoplasma arginini and Mycoplasma salivarium. 30 mAbs were obtained and one of these, designated CCM-2, was shown to bind to all five mycoplasma species. It also bound to Mycoplasma fermentans, Mycoplasma hominis and to all wild types (n = 54), isolated from cell cultures submitted to our laboratory. The mAb was used in a immunofluorescent assay (IF) and the method correlated with the microbiological assay. Using this mAb immunofluorescent staining of cells is a fast and simple procedure for mycoplasma detection in cell cultures.

摘要

在细胞培养物中检测支原体仍然是一个问题，尤其是在那些未将微生物检测和鉴定作为常规程序的实验室中。在我们实验室，已制备了针对莱氏无胆甾原体、猪鼻支原体、口腔支原体、精氨酸支原体和唾液支原体的单克隆抗体（mAb）。获得了30种单克隆抗体，其中一种命名为CCM-2，它被证明能与所有这五种支原体物种结合。它还与发酵支原体、人型支原体以及从提交到我们实验室的细胞培养物中分离出的所有野生型（n = 54）结合。该单克隆抗体用于免疫荧光测定（IF），该方法与微生物学测定相关。使用这种单克隆抗体对细胞进行免疫荧光染色是一种在细胞培养物中检测支原体的快速且简单的程序。