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在生产针对细胞内罗森尼亚菌的单克隆抗体时发生偶然污染后，生成针对支原体属的单克隆抗体。

Generation of a monoclonal antibody against Mycoplasma spp. following accidental contamination during production of a monoclonal antibody against Lawsonia intracellularis.

机构信息

Veterinary Research Center, Green Cross Veterinary Products Co., Ltd., Giheung-gu, Yongin-si, Republic of Korea.

出版信息

Appl Environ Microbiol. 2012 Mar;78(6):2046-8. doi: 10.1128/AEM.06705-11. Epub 2012 Jan 13.

DOI:10.1128/AEM.06705-11
PMID:22247145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3298139/
Abstract

This report describes Mycoplasma contamination of Lawsonia intracellularis cultures that led to the unintended acquisition of a monoclonal antibody against Mycoplasma spp. during the attempted generation of a monoclonal antibody against L. intracellularis.

摘要

本报告描述了支原体污染 Lawsonia intracellularis 培养物的情况，导致在试图生成针对 L. intracellularis 的单克隆抗体的过程中意外获得了一种针对支原体属的单克隆抗体。

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本文引用的文献

1
Generation and characterization of a monoclonal antibody with specificity for Mycoplasma arginini.具有针对精氨酸支原体特异性的单克隆抗体的产生与鉴定
J Microbiol. 2007 Dec;45(6):547-52.
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PCR-based detection of Mycoplasma species.基于聚合酶链反应的支原体物种检测。
J Microbiol. 2006 Feb;44(1):42-9.
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Comparison of intestinal mucosa homogenate and pure culture of the homologous Lawsonia intracellularis isolate in reproducing proliferative enteropathy in swine.猪肠道黏膜匀浆与同源胞内劳森菌分离株纯培养物在诱发猪增生性肠炎中的比较。
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Onset and duration of fecal shedding, cell-mediated and humoral immune responses in pigs after challenge with a pathogenic isolate or attenuated vaccine strain of Lawsonia intracellularis.用胞内劳森菌致病分离株或减毒疫苗株攻击猪后，粪便排毒的起始和持续时间、细胞介导免疫反应和体液免疫反应
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Elimination of mycoplasma from leukemia-lymphoma cell lines using antibiotics.使用抗生素清除白血病-淋巴瘤细胞系中的支原体。
Leukemia. 2002 Feb;16(2):284-8. doi: 10.1038/sj.leu.2402364.
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Proliferative enteropathy.增生性肠炎
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Intracellular bacteria of porcine proliferative enteropathy: cultivation and maintenance in vitro.猪增生性肠炎的胞内细菌：体外培养与维持
J Clin Microbiol. 1993 May;31(5):1136-42. doi: 10.1128/jcm.31.5.1136-1142.1993.
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Characterization of Lawsonia intracellularis gen. nov., sp. nov., the obligately intracellular bacterium of porcine proliferative enteropathy.猪增生性肠炎专性细胞内细菌——胞内劳森菌的新属及新种鉴定
Int J Syst Bacteriol. 1995 Oct;45(4):820-5. doi: 10.1099/00207713-45-4-820.
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Detection of mycoplasma contamination lymphoblastoid cell lines by monoclonal antibodies.用单克隆抗体检测淋巴母细胞系中的支原体污染
Hum Immunol. 1984 Feb;9(2):111-6. doi: 10.1016/0198-8859(84)90033-8.
10
Development and application of monoclonal antibodies for the detection of cell infecting mycoplasmas.用于检测感染细胞的支原体的单克隆抗体的研制与应用
J Immunol Methods. 1989 Nov 30;124(2):197-203. doi: 10.1016/0022-1759(89)90353-0.