Price Adam H
School of Biological Sciences, University of Aberdeen, Aberdeen AB24 3UU, UK.
Trends Plant Sci. 2006 May;11(5):213-6. doi: 10.1016/j.tplants.2006.03.006. Epub 2006 Apr 17.
It is generally believed that mapping quantitative trait loci (QTLs) does not accurately position genes underlying polygenic traits on the genome, which limits the application of QTL analysis in marker-assisted selection and gene discovery. However, now that a few plant QTLs have been cloned or accurately tagged, it appears that they might be accurate to within 2cM or less. This means that there will be circumstances when map-based cloning using only original mapping data would be a realistic option that avoids time-consuming and expensive fine mapping. Acceptance of this view would enhance the value of past and future mapping experiments, particularly those revealing small and environmentally sensitive QTLs that are often considered intractable at the molecular level.
人们普遍认为，定位数量性状基因座（QTL）并不能准确地在基因组上定位多基因性状的潜在基因，这限制了QTL分析在标记辅助选择和基因发现中的应用。然而，鉴于已经克隆或准确标记了一些植物QTL，似乎它们的定位精度可能在2厘摩或更低。这意味着在某些情况下，仅使用原始定位数据进行图位克隆将是一个现实的选择，可避免耗时且昂贵的精细定位。接受这一观点将提高过去和未来定位实验的价值，特别是那些揭示小的且对环境敏感的QTL的实验，这些QTL在分子水平上通常被认为难以处理。
