Xia Guiquan, Li Yuqi, Li Yu, Li Xuefei, Zhang Hua, Yu Yihua Bruce, Jiang Zhong-Xing
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, P. R. China.
Department of Pharmaceutical Sciences, University of Maryland, Baltimore, MD 21201, United States.
J Fluor Chem. 2014 Sep 1;164:39-42. doi: 10.1016/j.jfluchem.2014.06.005.
Using the set of fluorinated amphiles that contain the same fluorocarbon moiety but differ in their fluorine content percentage F% (25-45%), the optimal condition for a F%-based separation of these analytes using reverse-phase chromatography was explored. It is found that optimal separation can be achieved by pairing a regular reverse-phase column (such as C8) with a fluorinated eluent (such as trifluoroethanol). Separation is further improved at higher chromatographic temperature with baseline separation achieved at 45°C. This result indicates that the separation of fluorocarbon-tagged molecules can be based on the fluorine content percentage rather than the number of fluorine atoms.
使用一组含有相同碳氟化合物部分但氟含量百分比F%（25 - 45%）不同的氟化两亲物，探索了使用反相色谱基于F%分离这些分析物的最佳条件。发现通过将常规反相柱（如C8）与氟化洗脱剂（如三氟乙醇）配对可实现最佳分离。在较高的色谱温度下分离进一步改善，在45°C时实现基线分离。该结果表明，碳氟化合物标记分子的分离可基于氟含量百分比而非氟原子数量。
