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十二种固氮细菌的形态学、生物化学和分子特征及其对不同锌浓度的响应。

Morphological, biochemical and molecular characterization of twelve nitrogen-fixing bacteria and their response to various zinc concentration.

作者信息

Dadook Mohammad, Mehrabian Sedigheh, Salehi Mitra, Irian Saeed

机构信息

Faculty of Biological Science, Islamic Azad University Tehran North Branch, Tehran, IR Iran.

Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IR Iran.

出版信息

Jundishapur J Microbiol. 2014 Apr;7(4):e9415. doi: 10.5812/jjm.9415. Epub 2014 Apr 1.

DOI:10.5812/jjm.9415
PMID:25147702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4138622/
Abstract

BACKGROUND

Zinc is an essential micronutrient used in the form of zinc sulfate in fertilizers in the agriculture production system. Nitrogen-fixing microorganisms are also of considerable value in promoting soil fertility.

OBJECTIVES

This study aimed to investigate the degree of sensitivity to varying concentrations of zinc, in the form of ZnSO4, in different strains of Azotobacter chroococcum in a laboratory environment.

MATERIALS AND METHODS

To isolate A. chroococcum strains, soil samples were collected from wheat, corn and asparagus rhizospheres and cultured in media lacking nitrogen at 30˚C for 48 hours. Strains were identified based on morphological and biochemical characteristics. The presence of the nitrogenase enzyme system was confirmed by testing for the presence of the nifH gene using PCR analysis. The minimum inhibitory concentration (MIC) and optimal zinc concentration for the growth of each strain was determined.

RESULTS

A total of 12 bacterial strains were isolated from six different soil samples. A. chroococcum strains were morphologically and biochemically characterized. The presence of the nifH gene was confirmed in all the strains. MIC and the optimal zinc concentration for bacterial growth were 50 ppm and 20 ppm, respectively.

CONCLUSIONS

It was concluded that increasing the concentration of zinc in the agricultural soil is harmful to beneficial microorganisms and reduces the soil fertility. A 20-ppm zinc concentration in soil is suggested to be optimal.

摘要

背景

锌是一种必需的微量营养素，在农业生产系统的肥料中以硫酸锌的形式使用。固氮微生物在提高土壤肥力方面也具有重要价值。

目的

本研究旨在在实验室环境中，调查不同浓度硫酸锌形式的锌对不同菌株的褐球固氮菌的敏感程度。

材料与方法

为分离褐球固氮菌菌株，从小麦、玉米和芦笋根际采集土壤样本，并在缺乏氮的培养基中于30℃培养48小时。根据形态和生化特征对菌株进行鉴定。通过使用PCR分析检测nifH基因的存在来确认固氮酶系统的存在。确定每种菌株生长的最低抑菌浓度（MIC）和最佳锌浓度。

结果

从六个不同土壤样本中总共分离出12株细菌菌株。对褐球固氮菌菌株进行了形态和生化特征分析。在所有菌株中均确认了nifH基因的存在。细菌生长的MIC和最佳锌浓度分别为50 ppm和20 ppm。

结论

得出的结论是，增加农业土壤中锌的浓度对有益微生物有害，并会降低土壤肥力。建议土壤中锌的最佳浓度为20 ppm。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e591/4138622/4235b0ef1669/jjm-07-9415-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e591/4138622/8336564ec004/jjm-07-9415-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e591/4138622/c4954e50936f/jjm-07-9415-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e591/4138622/6d5643e063b0/jjm-07-9415-i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e591/4138622/4235b0ef1669/jjm-07-9415-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e591/4138622/8336564ec004/jjm-07-9415-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e591/4138622/c4954e50936f/jjm-07-9415-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e591/4138622/6d5643e063b0/jjm-07-9415-i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e591/4138622/4235b0ef1669/jjm-07-9415-g002.jpg

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