Kharami A, Bibi Z, Nielsen H, Høiby N, Döring G
Statens Seruminstitut, Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
APMIS. 1989 Dec;97(12):1068-72.
The effect of rhamnolipid purified from culture supernatant of Pseudomonas aeruginosa on human peripheral blood neutrophil and monocyte function was studied. It was shown that rhamnolipid at concentrations of up to 100 micrograms/ml did not affect neutrophil and monocyte chemotaxis towards various chemoattractants. The rhamnolipid by itself did not show chemotactic activity and did not induce any oxidative burst response. Preincubation of monocytes with rhamnolipid enhanced the oxidative burst response of these cells to phorbol myristate acetate (PMA) and to opsonized zymosan by 2 to 5 fold. The priming effect was observed both in a superoxide assay and in a chemiluminescence assay. However, rhamnolipid did not prime the neutrophil oxidative burst response. Monocytes/macrophages are involved in the inflammatory process in the lung infections caused by P. aeruginosa and oxygen radicals are known to cause tissue damage. Therefore the priming by rhamnolipid of monocytes for enhanced generation of oxygen radicals may play a role in the pathogenesis of tissue damage in the lungs of cystic fibrosis patients with P. aeruginosa infections.
研究了从铜绿假单胞菌培养上清液中纯化得到的鼠李糖脂对人外周血中性粒细胞和单核细胞功能的影响。结果表明，浓度高达100微克/毫升的鼠李糖脂不影响中性粒细胞和单核细胞对各种趋化因子的趋化作用。鼠李糖脂本身不显示趋化活性，也不诱导任何氧化爆发反应。用鼠李糖脂预孵育单核细胞可使这些细胞对佛波酯（PMA）和调理酵母聚糖的氧化爆发反应增强2至5倍。在超氧化物测定和化学发光测定中均观察到了这种启动效应。然而，鼠李糖脂不能启动中性粒细胞的氧化爆发反应。单核细胞/巨噬细胞参与了铜绿假单胞菌引起的肺部感染的炎症过程，并且已知氧自由基会导致组织损伤。因此，鼠李糖脂对单核细胞的启动作用以增强氧自由基的产生可能在患有铜绿假单胞菌感染的囊性纤维化患者肺部组织损伤的发病机制中起作用。
