高度保守的ENY2/Sus1蛋白与果蝇CTCF结合，是屏障活性所必需的。
Highly conserved ENY2/Sus1 protein binds to Drosophila CTCF and is required for barrier activity.
作者信息
Maksimenko Oksana, Kyrchanova Olga, Bonchuk Artem, Stakhov Viacheslav, Parshikov Alexander, Georgiev Pavel
机构信息
Laboratory of Gene Expression Regulation in Development; Institute of Gene Biology; Russian Academy of Sciences; Moscow, Russia.
Group of Transcriptional Regulation; Institute of Gene Biology; Russian Academy of Sciences; Moscow, Russia.
出版信息
Epigenetics. 2014 Sep;9(9):1261-70. doi: 10.4161/epi.32086. Epub 2014 Aug 1.
Chromatin insulators affect interactions between promoters and enhancers/silencers and function as barriers for the spreading of repressive chromatin. Drosophila insulator protein dCTCF marks active promoters and boundaries of many histone H3K27 trimethylation domains associated with repressed chromatin. In particular, dCTCF binds to such boundaries between the parasegment-specific regulatory domains of the Bithorax complex. Here we demonstrate that the evolutionarily conserved protein ENY2 is recruited to the zinc-finger domain of dCTCF and is required for the barrier activity of dCTCF-dependent insulators in transgenic lines. Inactivation of ENY2 by RNAi in BG3 cells leads to the spreading of H3K27 trimethylation and Pc protein at several dCTCF boundaries. The results suggest that evolutionarily conserved ENY2 is responsible for barrier activity mediated by the dCTCF protein.
染色质绝缘子影响启动子与增强子/沉默子之间的相互作用，并作为抑制性染色质扩散的屏障发挥作用。果蝇绝缘子蛋白dCTCF标记了许多与抑制性染色质相关的组蛋白H3K27三甲基化结构域的活性启动子和边界。特别是，dCTCF结合到双胸复合体节段特异性调控结构域之间的此类边界。在此，我们证明进化上保守的蛋白ENY2被招募到dCTCF的锌指结构域，并且是转基因系中dCTCF依赖性绝缘子的屏障活性所必需的。在BG3细胞中通过RNAi使ENY2失活会导致H3K27三甲基化和Pc蛋白在几个dCTCF边界处扩散。结果表明，进化上保守的ENY2负责由dCTCF蛋白介导的屏障活性。
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