Jabbari Azad Farahzad, Talaei Ali, Rafatpanah Houshang, Yousefzadeh Hadis, Jafari Rahele, Talaei Andishe, Farid Hosseini Reza
Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Psychiatry and Behavioral Sciences Research Center, School of Medicine, Mashhad University of Medical Sciences Mashhad, Iran.
Iran J Allergy Asthma Immunol. 2014 Dec;13(6):433-9.
The role of transforming growth factor (TGF)-β1, interferon (IFN)-γ, interleukin (IL)-2, IL-3, and IL-6 in the pathogenesis of Alzheimer's Disease (AD) has long been reported in literature. In this case-control study, the concentrations of these cytokines in altered T lymphocytes, as well as serum vitamin B12, have been compared in terms of factors such as, age, the clinical course and the patients' disease risk. 40 patients who met the DSM-IV-TR criteria of AD were selected and an age- and gender-matched control group was recruited. The participants' cognitive performance was measured according to the Mini Mental State Examination (MMSE), the Global Deterioration Scale (GDS) and Clinical Dementia Ratio (CDR). The levels of cytokines were measured in supernatants of lymphocytes culture, using assays of ELISA and atomic absorption. Higher levels of IL-6 and IFN-γ were found more in the altered T lymphocytes of the AD patients rather than in the control individuals. Furthermore, a marginal significant difference was found between the TGF-β levels of the two study groups. Regression analysis of CDR score and cytokines showed the inverse significant correlation between CDR score and IFN-γ levels. Furthermore, the relation between MMSE scores and IFN-γ was significant, meaning that by increasing MMSE score, IFN-γ level was significantly increased. This study suggests that the levels of IL-6 and IFN-γ are significantly increased in altered T lymphocytes of AD patients, as compared to those who are not inflicted with AD, and that they are related to the patient's age. Also, IFN-γ is related to the severity stage of the AD.
长期以来，文献中一直报道转化生长因子（TGF）-β1、干扰素（IFN）-γ、白细胞介素（IL）-2、IL-3和IL-6在阿尔茨海默病（AD）发病机制中的作用。在这项病例对照研究中，已根据年龄、临床病程和患者疾病风险等因素，比较了这些细胞因子在改变的T淋巴细胞中的浓度以及血清维生素B12的浓度。选择了40名符合AD的DSM-IV-TR标准的患者，并招募了一个年龄和性别匹配的对照组。根据简易精神状态检查表（MMSE）、总体衰退量表（GDS）和临床痴呆评定量表（CDR）测量参与者的认知表现。使用酶联免疫吸附测定（ELISA）和原子吸收测定法测量淋巴细胞培养上清液中的细胞因子水平。发现AD患者改变的T淋巴细胞中IL-6和IFN-γ的水平高于对照组个体。此外，两个研究组的TGF-β水平之间存在边缘显著差异。CDR评分与细胞因子的回归分析显示CDR评分与IFN-γ水平之间存在显著负相关。此外，MMSE评分与IFN-γ之间的关系显著，这意味着随着MMSE评分的增加，IFN-γ水平显著升高。本研究表明，与未患AD的患者相比，AD患者改变的T淋巴细胞中IL-6和IFN-γ的水平显著升高，且它们与患者年龄有关。此外，IFN-γ与AD的严重程度阶段有关。
