Clericò Vito, Masini Luca, Boni Adriano, Meucci Sandro, Cecchini Marco, Recchia Fabio A, Tredicucci Alessandro, Bifone Angelo
Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy; Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Pisa, Italy.
Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Pisa, Italy; NEST, CNR-Istituto Nanoscienze and Scuola Normale Superiore, Pisa, Italy.
PLoS One. 2014 Aug 25;9(8):e105474. doi: 10.1371/journal.pone.0105474. eCollection 2014.
Nanolithography techniques enable the fabrication of complex nanodevices that can be used for biosensing purposes. However, these devices are normally supported by a substrate and their use is limited to in vitro applications. Following a top-down procedure, we designed and fabricated composite inductance-capacitance (LC) nanoresonators that can be detached from their substrate and dispersed in water. The multimaterial composition of these resonators makes it possible to differentially functionalize different parts of the device to obtain stable aqueous suspensions and multi-sensing capabilities. For the first time, we demonstrate detection of these devices in an aqueous environment, and we show that they can be sensitized to their local environment and to chemical binding of specific molecular moieties. The possibility to optically probe the nanoresonator resonance in liquid dispersions paves the way to a variety of new applications, including injection into living organisms for in vivo sensing and imaging.
纳米光刻技术能够制造出可用于生物传感目的的复杂纳米器件。然而，这些器件通常由衬底支撑，其应用仅限于体外。按照自上而下的工艺，我们设计并制造了复合电感 - 电容（LC）纳米谐振器，它们可以从衬底上分离并分散在水中。这些谐振器的多材料组成使得对器件的不同部分进行差异化功能化成为可能，从而获得稳定的水性悬浮液和多传感能力。我们首次展示了在水性环境中对这些器件的检测，并表明它们能够对其局部环境以及特定分子部分的化学结合产生敏感响应。在液体分散体中对纳米谐振器共振进行光学探测的可能性为各种新应用铺平了道路，包括注入活生物体进行体内传感和成像。
