Lee L S
Southern Regional Research Center, New Orleans, LA 70179.
Mycopathologia. 1989 Sep;107(2-3):127-30. doi: 10.1007/BF00707549.
Non-aflatoxin-producing isolates of Aspergillus flavus from nature and isolates of A. flavus that had lost their toxigenic trait following laboratory transfer were compared biochemically. After the addition of aflatoxin B1 precursors sterigmatocystin or O-methylsterigmatocystin to whole cell cultures, the non-toxin producing isolates from nature remained non-toxigenic while toxigenicity was restored in the non-toxigenic laboratory strains. Results imply a lack of enzymes needed for biochemical conversions of precursors to aflatoxin B1 in natural non-producers and suppression of these enzymes in the non-producing laboratory strains.
对来自自然界的不产黄曲霉毒素的黄曲霉菌株以及经实验室传代后丧失产毒特性的黄曲霉菌株进行了生化比较。在向全细胞培养物中添加黄曲霉毒素B1前体柄曲霉素或O-甲基柄曲霉素后，来自自然界的不产毒素菌株仍不产毒，而不产毒的实验室菌株恢复了产毒能力。结果表明，天然不产毒菌株缺乏将前体生化转化为黄曲霉毒素B1所需的酶，而不产毒的实验室菌株中这些酶受到抑制。
