Henderberg A, Bennett J W, Lee L S
Department of Biology, Tulane University, New Orleans, Louisiana 70118.
J Gen Microbiol. 1988 Mar;134(3):661-7. doi: 10.1099/00221287-134-3-661.
The origin of aflatoxin G1 was studied using mutant strains of Aspergillus parasiticus blocked early in the pathway and by tracing 14C-labelled aflatoxin B1 (AFB1) in wild-type A. flavus and A. parasiticus strains. Sterigmatocystin (ST) was a precursor of AFB1, AFG1 and AFG2 in the four mutants examined. The identity of AFG1 was confirmed by mass spectrometry. No evidence for conversion of AFB1 to AFG1 was found. A rigorously controlled study of conversions of radioactivity based on preparative thin-layer chromatography of aflatoxins demonstrated that low levels of aflatoxin interconversions previously reported in the literature might actually be artifacts.
利用在该途径早期受阻的寄生曲霉突变菌株，并通过追踪野生型黄曲霉和寄生曲霉菌株中14C标记的黄曲霉毒素B1（AFB1），对黄曲霉毒素G1的起源进行了研究。在所检测的四个突变体中，柄曲霉素（ST）是AFB1、AFG1和AFG2的前体。通过质谱法确认了AFG1的身份。未发现AFB1转化为AFG1的证据。基于黄曲霉毒素制备薄层色谱法对放射性转化进行的严格对照研究表明，文献中先前报道的低水平黄曲霉毒素相互转化可能实际上是人为现象。
