Gaur U, Xiong Y Y, Luo Q P, Yuan F Y, Wu H Y, Qiao M, Wimmers K, Li K, Mei S Q, Liu G S
Institute of Animal Science and Veterinary Medicine, Hubei Academy of Agricultural Sciences, Yaoyuan No. 1, Nanhu, Hongshan District, Wuhan, 430064, China.
Mol Biol Rep. 2014 Dec;41(12):7865-73. doi: 10.1007/s11033-014-3680-x. Epub 2014 Aug 27.
Different pig breeds have shown differential susceptibility to the pathogen infection; however, molecular mechanisms of the infection susceptibility are not fully understood. Streptococcus suis type 2 (SS2) is an important zoonotic pathogen. To identify the genes responsible for infection susceptibility, pigs from two different breeds (Enshi black and Landrace) were inoculated with SS2 and their spleen transcriptome profiles were investigated in the present study. The differentially expressed genes (DEGs) were analyzed from infected versus control pigs in each breed, and then compared between both pig breeds. Enshi black pig showed more DEGs than Landrace (830 vs. 611) and most of these were due to down-regulated genes (543 vs. 387). However some DEGs were uniquely expressed in one breed, some were expressed in opposite direction in both breeds. A number of candidate genes and pathways are identified which might be involved in susceptibility to SS2, for example, MMP9 and Resistin were only significantly expressed in Landrace. NPG3 and PMAP23 were up-regulated in Landrace whereas down-regulated in Enshi black. LENG8 in control Landrace have inherently higher expression than control Enshi black. IGKV6 is down-regulated in Landrace but up-regulated in Enshi black. Overall, the transcriptome profiles are consistent with the clinical signs, i.e. the Enshi black is more susceptible to SS2 infection than Landrace. This is the first study to identify differential gene expression between indigenous and modern commercial pigs after in vivo SS2 infection using RNA-seq. The significant DEGs in splenic profiles between two pig breeds suggested considerable involvement of genetic background in susceptibility to the SS2 infection in pigs.
不同猪种对病原体感染表现出不同的易感性；然而，感染易感性的分子机制尚未完全明确。猪链球菌2型（SS2）是一种重要的人畜共患病原体。为了鉴定与感染易感性相关的基因，本研究对两个不同猪种（恩施黑猪和长白猪）接种SS2，并对其脾脏转录组图谱进行了研究。分别分析了每个猪种中感染猪与对照猪之间的差异表达基因（DEG），然后对两个猪种进行比较。恩施黑猪的DEG比长白猪多（830个对611个），且大多数是下调基因（543个对387个）。然而，一些DEG只在一个猪种中特异性表达，一些在两个猪种中表达方向相反。鉴定出了一些可能与SS2易感性有关的候选基因和通路，例如，基质金属蛋白酶9（MMP9）和抵抗素仅在长白猪中显著表达。NPG3和PMAP23在长白猪中上调而在恩施黑猪中下调。对照长白猪中的LENG8固有表达高于对照恩施黑猪。IGKV6在长白猪中下调但在恩施黑猪中上调。总体而言转录组图谱与临床症状一致，即恩施黑猪比长白猪更易感染SS2。这是第一项利用RNA测序鉴定体内感染SS2后本地猪和现代商业猪之间差异基因表达的研究。两个猪种脾脏图谱中的显著DEG表明遗传背景在猪对SS2感染的易感性中起重要作用。
