Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
SPKLOMHNM and Central Laboratory, Zhong Yuan Academy of Biological Medicine, Liaocheng University, Liaocheng People's Hospital, Liaocheng, Shandong 252000, PR China.
J Chromatogr A. 2014 Oct 3;1362:89-101. doi: 10.1016/j.chroma.2014.08.028. Epub 2014 Aug 14.
Shenfu decoction (SFD) is a well-known important traditional Chinese medicine prescription that has been used to treat chronic heart failure (CHF) for many years in China. However, its holistic therapeutic effects and mechanism are not yet well understood. Here, a UPLC/TOF-MS-based metabonomic study was conducted to explore potential biomarkers to increase the understanding of CHF and to assess the integral efficacy of SFD for CHF in rats. Principal component analysis was used to investigate the global metabonomic alterations and to evaluate the therapeutic effects of SFD in rats. Clear separations were observed not only in the comparison of dynamic trajectories but also in the comparison of the metabolite profiles of the normal controls, the model group (CHF), the sham surgery group and the SFD-treated group. The results indicated that SFD exerted therapeutic efficacies for CHF, which were in accordance with the results of the echocardiographic and haemodynamic assay. Furthermore, 16 potential biomarkers from urine samples and 13 potential biomarkers from serum samples of CHF rats were identified and were mainly related to inflammation and to dysfunction of amino acids and energy metabolism. The therapeutic effects of SFD on CHF were partially due to the restoration of these disturbed pathways. This study provides a useful approach for exploring the mechanism of myocardial infarction (MI)-induced CHF and for evaluating the efficacy of SFD on CHF.
参附汤（SFD）是一种著名的重要中药方剂，在中国已用于治疗慢性心力衰竭（CHF）多年。然而，其整体治疗效果和机制尚不清楚。在这里，进行了基于 UPLC/TOF-MS 的代谢组学研究，以探索潜在的生物标志物，以增加对 CHF 的理解，并评估 SFD 对 CHF 大鼠的整体疗效。主成分分析用于研究全局代谢组学变化，并评估 SFD 在大鼠中的治疗效果。不仅在正常对照组、模型组（CHF）、假手术组和 SFD 治疗组之间的动态轨迹比较中，而且在代谢物图谱的比较中，都观察到了明显的分离。结果表明，SFD 对 CHF 具有治疗作用，这与超声心动图和血流动力学测定的结果一致。此外，从 CHF 大鼠的尿液样本中鉴定出 16 个潜在的生物标志物，从血清样本中鉴定出 13 个潜在的生物标志物，这些生物标志物主要与炎症以及氨基酸和能量代谢功能障碍有关。SFD 对 CHF 的治疗作用部分归因于这些受干扰途径的恢复。本研究为探讨心肌梗死（MI）诱导的 CHF 的机制以及评估 SFD 对 CHF 的疗效提供了一种有用的方法。
