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代谢组学图谱描绘了中药四逆汤对心肌梗死大鼠的影响。

Metabonomic profiles delineate the effect of traditional Chinese medicine sini decoction on myocardial infarction in rats.

机构信息

School of Pharmacy, Second Military Medical University, Shanghai, China.

出版信息

PLoS One. 2012;7(4):e34157. doi: 10.1371/journal.pone.0034157. Epub 2012 Apr 6.

DOI:10.1371/journal.pone.0034157
PMID:22493681
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3320902/
Abstract

BACKGROUND

In spite of great advances in target-oriented Western medicine for treating myocardial infarction (MI), it is still a leading cause of death in a worldwide epidemic. In contrast to Western medicine, Traditional Chinese medicine (TCM) uses a holistic and synergistic approach to restore the balance of Yin-Yang of body energy so the body's normal function can be restored. Sini decoction (SND) is a well-known formula of TCM which has been used to treat MI for many years. However, its holistic activity evaluation and mechanistic understanding are still lacking due to its complex components.

METHODOLOGY/PRINCIPAL FINDINGS: A urinary metabonomic method based on nuclear magnetic resonance and ultra high-performance liquid chromatography coupled to mass spectrometry was developed to characterize MI-related metabolic profiles and delineate the effect of SND on MI. With Elastic Net for classification and selection of biomarkers, nineteen potential biomarkers in rat urine were screened out, primarily related to myocardial energy metabolism, including the glycolysis, citrate cycle, amino acid metabolism, purine metabolism and pyrimidine metabolism. With the altered metabolism pathways as possible drug targets, we systematically analyze the therapeutic effect of SND, which demonstrated that SND administration could provide satisfactory effect on MI through partially regulating the perturbed myocardial energy metabolism.

CONCLUSIONS/SIGNIFICANCE: Our results showed that metabonomic approach offers a useful tool to identify MI-related biomarkers and provides a new methodological cue for systematically dissecting the underlying efficacies and mechanisms of TCM in treating MI.

摘要

背景

尽管靶向治疗心肌梗死（MI）的西方医学取得了巨大进展，但它仍然是全球范围内的主要死亡原因。与西方医学不同，中医（TCM）采用整体和协同的方法来恢复身体能量的阴阳平衡，从而恢复身体的正常功能。四逆汤（SND）是一种著名的中药方剂，多年来一直用于治疗 MI。然而，由于其复杂的成分，其整体活性评价和机制理解仍然缺乏。

方法/主要发现：本研究建立了一种基于核磁共振和超高效液相色谱-质谱联用的尿代谢组学方法，用于描述 MI 相关的代谢谱，并阐明 SND 对 MI 的作用。通过弹性网络进行分类和生物标志物选择，从大鼠尿液中筛选出 19 个潜在的生物标志物，主要与心肌能量代谢有关，包括糖酵解、柠檬酸循环、氨基酸代谢、嘌呤代谢和嘧啶代谢。结合可能的药物靶点，系统分析 SND 的治疗效果，结果表明 SND 通过部分调节心肌能量代谢紊乱，对 MI 具有令人满意的治疗效果。

结论/意义：本研究结果表明，代谢组学方法为识别 MI 相关生物标志物提供了一种有用的工具，并为系统剖析 TCM 治疗 MI 的潜在疗效和机制提供了一种新的方法线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3415/3320902/632d3d3e2aa4/pone.0034157.g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3415/3320902/632d3d3e2aa4/pone.0034157.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3415/3320902/701ddcc0c9e7/pone.0034157.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3415/3320902/db868a873d75/pone.0034157.g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3415/3320902/498fa9f8b0fa/pone.0034157.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3415/3320902/8ae31e83b97b/pone.0034157.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3415/3320902/59a2e578e315/pone.0034157.g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3415/3320902/632d3d3e2aa4/pone.0034157.g009.jpg

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