Garcia Valeria S, Gonzalez Verónica D G, Marcipar Ivan S, Gugliotta Luis M
INTEC (Universidad Nacional del Litoral and CONICET), Santa Fe, Argentina.
Trop Med Int Health. 2014 Nov;19(11):1346-54. doi: 10.1111/tmi.12379. Epub 2014 Aug 30.
To evaluate the diagnostic performance of novel latex-protein complexes obtained from different antigens of Trypanosoma cruzi through immunoagglutination test using a panel of T. cruzi-positive sera, leishmaniasis-positive sera and negative sera for both parasites.
Complexes' behaviour using total parasite homogenate (TPH), two simple recombinant proteins (RP1 and RP5) and two chimeric recombinant proteins (CP1 and CP2) was comparatively evaluated. The area under ROC curves was used as an index of accuracy. Sensitivity, specificity and discrimination efficiency were assessed.
All recombinant antigens showed higher specificity than TPH. The lower specificity of TPH was mainly due to cross-reacting peptides between T. cruzi and Leishmania spp. In turn, all performance indicators were higher for CP1 and CP2 than for RP1 and RP5. The carboxylated latex-CP2 (C2-CP2) complex was able to detect antibodies against T. cruzi. The values of area under ROC curve (0.96), sensitivity (92.3%, 95% CI: 79.4-100.0%) and specificity (84.0%, 95% CI: 67.6-100.0%) indicate that the assay could be used as a screening test.
The C2-CP2 complex could be an important tool to carry out sero-epidemiological studies.
使用一组克氏锥虫阳性血清、利什曼病阳性血清以及两种寄生虫均为阴性的血清，通过免疫凝集试验评估从克氏锥虫不同抗原获得的新型乳胶 - 蛋白质复合物的诊断性能。
比较评估了使用全寄生虫匀浆（TPH）、两种简单重组蛋白（RP1和RP5）以及两种嵌合重组蛋白（CP1和CP2）时复合物的表现。ROC曲线下面积用作准确性指标。评估了敏感性、特异性和鉴别效率。
所有重组抗原均显示出比TPH更高的特异性。TPH较低的特异性主要归因于克氏锥虫和利什曼原虫属之间的交叉反应肽。反过来，CP1和CP2的所有性能指标均高于RP1和RP5。羧化乳胶 - CP2（C2 - CP2）复合物能够检测抗克氏锥虫抗体。ROC曲线下面积值（0.96）、敏感性（92.3%，95%CI：79.4 - 100.0%）和特异性（84.0%，95%CI：67.6 - 100.0%）表明该检测可作为一种筛查试验。
C2 - CP2复合物可能是开展血清流行病学研究的重要工具。
