皮质中间神经元的亚型选择性电穿孔
Subtype-selective electroporation of cortical interneurons.
作者信息
De Marco Garcia Natalia V, Fishell Gord
机构信息
NYU Neuroscience Institute, New York University School of Medicine; Brain and Mind Research Institute, Weill Cornell Medical College;
NYU Neuroscience Institute, New York University School of Medicine.
出版信息
J Vis Exp. 2014 Aug 18(90):e51518. doi: 10.3791/51518.
The study of central nervous system (CNS) maturation relies on genetic targeting of neuronal populations. However, the task of restricting the expression of genes of interest to specific neuronal subtypes has proven remarkably challenging due to the relative scarcity of specific promoter elements. GABAergic interneurons constitute a neuronal population with extensive genetic and morphological diversity. Indeed, more than 11 different subtypes of GABAergic interneurons have been characterized in the mouse cortex. Here we present an adapted protocol for selective targeting of GABAergic populations. We achieved subtype selective targeting of GABAergic interneurons by using the enhancer element of the homeobox transcription factors Dlx5 and Dlx6, homologues of the Drosophila distal-less (Dll) gene, to drive the expression of specific genes through in utero electroporation.
中枢神经系统（CNS）成熟的研究依赖于对神经元群体的基因靶向。然而，由于特定启动子元件相对稀缺，将感兴趣的基因表达限制在特定神经元亚型上的任务已被证明极具挑战性。γ-氨基丁酸（GABA）能中间神经元构成了一个具有广泛遗传和形态多样性的神经元群体。事实上，在小鼠皮层中已鉴定出超过11种不同亚型的GABA能中间神经元。在此，我们提出一种适用于选择性靶向GABA能群体的方案。我们通过使用同源异形框转录因子Dlx5和Dlx6（果蝇远端缺失（Dll）基因的同源物）的增强子元件，通过子宫内电穿孔驱动特定基因的表达，实现了对GABA能中间神经元的亚型选择性靶向。
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