Coltharp Carla, Yang Xinxing, Xiao Jie
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Curr Opin Struct Biol. 2014 Oct;28:112-21. doi: 10.1016/j.sbi.2014.08.008. Epub 2014 Aug 30.
This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10-50 nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images.
本综述重点介绍了基于单分子定位的超分辨率成像方法的定量能力。除了揭示精细的结构细节外,这些方法生成的分子坐标列表还提供了以10 - 50纳米分辨率量化分子数量、聚集和共定位的关键能力。在这里,我们描述了单分子超分辨率图像定量分析的典型工作流程和注意事项。这些指南包括潜在的陷阱和必要的对照实验,以便对超分辨率图像进行严格的评估和解读。