土耳其特有的披针叶黄华各种提取物对牙周病原菌的抗菌活性、抗氧化及细胞毒性作用
Antimicrobial activity against periodontopathogenic bacteria, antioxidant and cytotoxic effects of various extracts from endemic Thermopsis turcica.
作者信息
Bali Elif Burcu, Açık Leyla, Akca Gülçin, Sarper Meral, Elçi Mualla Pınar, Avcu Ferit, Vural Mecit
机构信息
Health Services Vocational School, University of Gazi, Ankara, Turkey.
Department of Biology, Faculty of Science, University of Gazi, Ankara, Turkey.
出版信息
Asian Pac J Trop Biomed. 2014 Jul;4(7):505-14. doi: 10.12980/APJTB.4.2014APJTB-2013-0010.
OBJECTIVE
To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan, Vural & Küçüködük against periodontopathogenic bacteria, its antioxidant activity and cytotoxic effect on various cancer cell lines.
METHODS
In vitro antimicrobial activities of ethanol, methanol, ethyl acetate (EtAc), n-hexane and water extracts of Thermopsis turcica herb against periodontopathogenic bacteria, Aggregatibacter actinomycetemcomitans ATCC 29523 and Porphyromonas gingivalis ATCC 33277 were tested by agar well diffusion, minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Antioxidant properties of the extracts were evaluated by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity and β-carotene bleaching methods. Amounts of phenolic contents of the extracts were also analysed by using the Folin-Ciocalteu reagent. Additionally, cytotoxic activity of the extracts on androgen-insensitive prostate cancer, androgen-sensitive prostate cancer, chronic myelogenous leukemia and acute promyelocytic leukemia human cancer cell lines were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Human gingival fibroblast cells were used as a control.
RESULTS
Our data showed that EtAc extract had the highest antimicrobial effect on Aggregatibacter actinomycetemcomitans (MIC: 1.562 mg/mL, MBC: 3.124 mg/mL) and Porphyromonas gingivalis (MIC: 0.781 mg/mL, MBC: 1.562 mg/mL). In antioxidant assays, EtAc extract exhibited also the highest radical scavenging activity [IC50=(30.0±0.3) µg/mL] and the highest inhibition [(74.35±0.30)%] against lineloic acide oxidation. The amount of phenolic content of it was also the highest [(162.5±1.2) µg/mg gallic acid]. In cytotoxic assay, only ethanol [IC50=(80.00±1.21) µg/mL] and EtAc extract [IC50=(70.0±0.9) µg/mL] were toxic on acute promyelocytic leukemia cells at 20-100 µg/mL (P<0.05). However, no toxic effect was observed on human gingival fibroblast cells.
CONCLUSIONS
According to our findings, owing to its antioxidant and cytotoxic potential, EtAc extract might include anticancer agents for acute promyelocytic leukemia.
目的
研究土耳其披针叶黄华（Thermopsis turcica Kit Tan、Vural & Küçüködük）对牙周病原菌的体外抗菌潜力、其抗氧化活性以及对多种癌细胞系的细胞毒性作用。
方法
采用琼脂孔扩散法、最低抑菌浓度（MIC）和最低杀菌浓度（MBC），检测土耳其披针叶黄华草的乙醇、甲醇、乙酸乙酯（EtAc）、正己烷和水提取物对牙周病原菌伴放线聚集杆菌ATCC 29523和牙龈卟啉单胞菌ATCC 33277的体外抗菌活性。通过1,1-二苯基-2-苦基肼自由基清除活性和β-胡萝卜素漂白法评估提取物的抗氧化性能。还使用福林-酚试剂分析提取物中酚类物质的含量。此外，通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法测定提取物对雄激素不敏感前列腺癌、雄激素敏感前列腺癌、慢性粒细胞白血病和急性早幼粒细胞白血病人类癌细胞系的细胞毒性活性。以人牙龈成纤维细胞作为对照。
结果
我们的数据表明，乙酸乙酯提取物对伴放线聚集杆菌（MIC：1.562 mg/mL，MBC：3.124 mg/mL）和牙龈卟啉单胞菌（MIC：0.781 mg/mL，MBC：1.562 mg/mL）具有最高的抗菌效果。在抗氧化试验中，乙酸乙酯提取物还表现出最高的自由基清除活性[IC50 =(30.0±0.3) µg/mL]和对亚油酸氧化的最高抑制率[(74.35±0.30)%]。其酚类物质含量也最高[(162.5±1.2) µg/mg没食子酸]。在细胞毒性试验中，仅乙醇[IC50 =(80.00±1.21) µg/mL]和乙酸乙酯提取物[IC50 =(70.0±0.9) µg/mL]在20 - 100 µg/mL时对急性早幼粒细胞白血病细胞有毒性（P<0.05）。然而，在人牙龈成纤维细胞上未观察到毒性作用。
结论
根据我们的研究结果，由于其抗氧化和细胞毒性潜力，乙酸乙酯提取物可能包含用于急性早幼粒细胞白血病的抗癌剂。
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