Firuzi Omidreza, Miri Ramin, Asadollahi Mojtaba, Eslami Saba, Jassbi Amir Reza
Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Pharm Res. 2013 Fall;12(4):801-10.
The plants of the genus Salvia synthesize several types of secondary metabolites with antimicrobial, cytotoxic, and radical scavenging activities and are used in the folk medicine of different countries. Eleven Salvia species including S. aegyptiaca, S. aethiopis, S. atropatana, S. eremophila, S. hypoleuca, S. limbata, S. nemorosa, S. santolinifolia, S. sclarea, S. syriaca, and S. xanthocheila were collected from different localities in Iran and screened for their cytotoxic activity using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The antioxidant potential and total phenol contents of the plant extracts were assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and Folin- Ciocalteu reagent respectively and finally antimicrobial activity of the above extracts were determined by using agar disc diffusion (ADD) and nutrient broth micro-dilution (NBMD) bioassays. Cytotoxic activity of methanol, 80% methanol and dichloromethane extracts of these plants were assessed on 3 human cancer cell lines. All of the extracts of S. eremophila and S. santolinifolia were active at IC50 values of 10.5-75.2 μg extract/mL, while the methanol and dichloromethane extracts of S. limbata, S. hypoleuca and S. aethiopis showed considerable cytotoxic activity against the tested cell lines. Among the tested plants for their antioxidant activity, S. nemorosa, S. atropatana, S. santolinifolia, and S. eremophila were the most active radical scavengers with higher total phenol contents while, S. limbata, S. xanthocheila and S. aegyptiaca were the weakest ones. The methanol extracts of S. santolinifolia, S. eremophila, S. sclarea and S. limbata inhibited the growth of all tested bacterial strains.
鼠尾草属植物能合成多种具有抗菌、细胞毒性和自由基清除活性的次生代谢产物，在不同国家的民间医学中都有应用。从伊朗不同地区采集了11种鼠尾草，包括埃及鼠尾草、埃塞俄比亚鼠尾草、阿特罗帕坦鼠尾草、旱生鼠尾草、浅色鼠尾草、具缘鼠尾草、林地鼠尾草、圣托里尼鼠尾草、鼠尾草、叙利亚鼠尾草和黄唇鼠尾草，并采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）比色法筛选它们的细胞毒性活性。分别通过2,2-二苯基-1-苦基肼（DPPH）自由基清除试验和福林-酚试剂评估植物提取物的抗氧化潜力和总酚含量，最后通过琼脂平板扩散（ADD）和营养肉汤微量稀释（NBMD）生物测定法测定上述提取物的抗菌活性。对这11种植物的甲醇、80%甲醇和二氯甲烷提取物在3种人类癌细胞系上评估细胞毒性活性。旱生鼠尾草和圣托里尼鼠尾草的所有提取物在IC50值为10.5 - 75.2 μg提取物/mL时具有活性，而具缘鼠尾草、浅色鼠尾草和埃塞俄比亚鼠尾草的甲醇和二氯甲烷提取物对测试细胞系显示出相当的细胞毒性活性。在所测试的具有抗氧化活性的植物中，林地鼠尾草、阿特罗帕坦鼠尾草、圣托里尼鼠尾草和旱生鼠尾草是最活跃的自由基清除剂，总酚含量较高，而具缘鼠尾草、黄唇鼠尾草和埃及鼠尾草是最弱的。圣托里尼鼠尾草、旱生鼠尾草、鼠尾草和具缘鼠尾草的甲醇提取物抑制了所有测试细菌菌株的生长。
