Picart Pere, Müller Christoph, Mottweiler Jakob, Wiermans Lotte, Bolm Carsten, Domínguez de María Pablo, Schallmey Anett
Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen (Germany).
ChemSusChem. 2014 Nov;7(11):3164-71. doi: 10.1002/cssc.201402465. Epub 2014 Sep 3.
Microbial β-etherases, which selectively cleave the β-O-4 aryl ether linkage present in lignin, hold great promise for future applications in lignin valorization. However, very few members have been reported so far and little is known about these enzymes. By using a database mining approach, four novel bacterial β-etherases were identified, recombinantly produced in Escherichia coli, and investigated together with known β-etherases in the conversion of various lignin and non-lignin-type model compounds. The resulting activities revealed the significant influence of the substituents at the phenyl ring adjacent to the ether bond. Finally, β-etherase activity on polymeric substrates, measured by using a fluorescently labeled synthetic lignin, was also proven; this underlined the applicability of the enzymes for the conversion of lignin into renewable chemicals.
微生物β-醚酶能够选择性地裂解木质素中存在的β-O-4芳基醚键，在木质素增值的未来应用中具有巨大潜力。然而，到目前为止，报道的成员非常少，对这些酶的了解也很少。通过数据库挖掘方法，鉴定出了四种新型细菌β-醚酶，在大肠杆菌中进行重组表达，并与已知的β-醚酶一起研究了它们对各种木质素和非木质素型模型化合物的转化作用。所得活性揭示了与醚键相邻的苯环上取代基的显著影响。最后，还证明了使用荧光标记的合成木质素测量的聚合物底物上的β-醚酶活性；这突出了这些酶在将木质素转化为可再生化学品方面的适用性。
