Stegmann Evi, Albersmeier Andreas, Spohn Marius, Gert Helena, Weber Tilmann, Wohlleben Wolfgang, Kalinowski Jörn, Rückert Christian
Mikrobiologie und Biotechnologie, Interfakultäres Institut für Mikrobiologie und Infektionsmedizin/Deutsches Zentrum für Infektionsforschung, Universität Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
Technology Platform Genomics, Center for Biotechnology (CeBiTec), Bielefeld University, Sequenz 1, 33615 Bielefeld, Germany.
J Biotechnol. 2014 Nov 10;189:46-7. doi: 10.1016/j.jbiotec.2014.08.034. Epub 2014 Sep 3.
We report the complete genome sequence of Amycolatopsis japonica MG417-CF17(T) (=DSM 44213(T)) which was identified as the producer of (S,S)-N,N'-ethylenediaminedisuccinic acid during a screening for phospholipase C inhibitors. The genome of A. japonica MG417-CF17(T) consists of two replicons: the chromosome (8,961,318 bp, 68.89% G+C content) and the plasmid pAmyja1 (92,539 bp, 68.23% G+C content), encoding a total of 8422 protein coding genes. Analysis of the sequence data revealed 30 clusters encoding the biosynthesis of secondary metabolites.
我们报道了日本拟无枝酸菌MG417-CF17(T)（=DSM 44213(T)）的全基因组序列，该菌株在磷脂酶C抑制剂筛选过程中被鉴定为(S,S)-N,N'-乙二胺二琥珀酸的产生菌。日本拟无枝酸菌MG417-CF17(T)的基因组由两个复制子组成：染色体（8,961,318 bp，G+C含量为68.89%）和质粒pAmyja1（92,539 bp，G+C含量为68.23%），共编码8422个蛋白质编码基因。序列数据分析显示有30个编码次生代谢物生物合成的基因簇。
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