Pankovics Péter, Boros Ákos, Kiss Tamás, Reuter Gábor
Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Szabadság út 7, 7623, Pécs, Hungary.
Arch Virol. 2015 Jan;160(1):345-51. doi: 10.1007/s00705-014-2228-7. Epub 2014 Sep 7.
The genus Kobuvirus (Picornaviridae) consists of three species, Aichivirus A (e.g., Aichi virus, which infects humans), Aichivirus B and Aichivirus C. Kobuvirus have not been detected in non-mammal species including birds. In this study, a novel kobuvirus was identified in 3 (17 %) out of 18 faecal samples collected from European rollers (Coracias garrulus) in Hungary. The complete genome sequence of strain SZAL6-KoV/2011/HUN (KJ934637), which was determined using a novel 5'/3' RACE method (dsRNA-RACE) involving a double-stranded (ds)RNA intermediate, has a type-V IRES at the 5' end and a cis-acting element (CRE) in the 3C gene and encodes L and 2A(H-box/NC) proteins, but it does not contain the sequence forming a "barbell-like" secondary RNA structure in the 3'UTR. SZAL6-KoV/2011/HUN has 72 %, 73 %, and 81 % amino acid sequence identity to the P1, P2, and P3 protein, respectively, of Aichi virus. Evolutionary analysis showed that SZAL6-KoV/2011/HUN shares a common ancestor with other kobuviruses but belongs to a more ancient lineage in the species Aichivirus A. Investigation of the known kobuviruses in different animals and discovery of novel kobuviruses in potential host species helps to clarify the evolutionary connection and zoonotic potential of kobuviruses.
杯状病毒属（小RNA病毒科）由三个种组成，即艾奇病毒A（如感染人类的爱知病毒）、艾奇病毒B和艾奇病毒C。在包括鸟类在内的非哺乳动物物种中未检测到杯状病毒。在本研究中，从匈牙利的欧洲佛法僧（佛法僧属）采集的18份粪便样本中，有3份（17%）鉴定出一种新型杯状病毒。使用一种涉及双链（ds）RNA中间体的新型5'/3' RACE方法（dsRNA-RACE）测定的菌株SZAL6-KoV/2011/HUN（KJ934637）的完整基因组序列，在5'端具有V型内部核糖体进入位点（IRES），在3C基因中有一个顺式作用元件（CRE），并编码L和2A（H-box/NC）蛋白，但在3'非翻译区（UTR）中不包含形成“杠铃状”二级RNA结构的序列。SZAL6-KoV/2011/HUN与爱知病毒的P1、P2和P3蛋白的氨基酸序列同一性分别为72%、73%和81%。进化分析表明，SZAL6-KoV/2011/HUN与其他杯状病毒有共同的祖先，但属于艾奇病毒A种中更古老的谱系。对不同动物中已知杯状病毒的研究以及在潜在宿主物种中发现新型杯状病毒有助于阐明杯状病毒的进化联系和人畜共患病潜力。
